FK 3311
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
FK 3311是一种具有细胞透过性的可口服的磺酰,对环氧酶(COX)-2具有高选择性抑制,其IC50值为1.6 μM。
环氧酶(COX)-2是一种膜结合蛋白,将电子从细胞色素c转移到催化亚基1。COX-2的表达受多种介质的紧密调节和诱导,如细胞因子、生长因子和内毒素。
FK 3311选择性抑制人血小板和单核细胞中组成型和诱导型COX-2的活性[1]。
此化合物也广泛的用于各种动物模型中以研究COX-2的功能。在心肌缺血再灌注大鼠模型中,通过阴茎静脉给予FK 3311能显著降低血清中天冬氨酸转氨酶(AST)、乳酸脱氢酶(LDH)、丙氨酸转氨酶(ALT)和凝血恶烷(Tx) B2的水平。但是,FK 3311处理并没有降低大鼠体内6-酮-PG F1a水平[2]。另外,相较于对照组,FK 3311处理的大鼠肝组织血流量要显著改善。肝脏的组织学检验表明FK 3311处理能够降低肝组织损伤,并提高移植肝功能[3]。在犬肺移植模型中,FK 3311处理能够降低肺损伤、中性粒细胞肺浸润、锝-99m-白蛋白积累以及凝血恶烷B2的水平。FK 3311处理后的肺具有更好的肺部气体交换和血液动力学[4]。
参考文献:
1. Grossman CJ, Wiseman J, Lucas FS, Trevethick MA, Birch PJ. Inhibition of constitutive and inducible cyclooxygenase activity in human platelets and mononuclear cells by NSAIDs and Cox 2 inhibitors. Inflamm Res 1995,44:253-257.
2. Kobayashi M, Takeyoshi I, Kurabayashi M, Matsumoto K, Morishita Y. The effects of a cyclooxygenase-2 inhibitor, FK3311, on total hepatic ischemia-reperfusion injury of the rat. Hepatogastroenterology 2007,54:522-526.
3. Oshima K, Yabata Y, Yoshinari D, Takeyoshi I. The effect of cyclooxygenase (COX)-2 inhibition on ischemia-reperfusion injury in liver transplantation. J Invest Surg 2009,22:239-245.
4. Sunose Y, Takeyoshi I, Tsutsumi H, Ohwada S, Oriuchi N, Matsumoto K, et al. Effect of a cyclooxygenase-2 inhibitor, FK3311, in a canine lung transplantation model. Ann Thorac Surg 2001,72:1165-1171; discussion 1171-1162.
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 341.33 |
Cas No. | 116686-15-8 |
Formula | C15H13F2NO4S |
Synonyms | FK-3311;FK3311 |
Solubility | Soluble in DMSO |
Chemical Name | N-[4-acetyl-2-(2,4-difluorophenoxy)phenyl]methanesulfonamide |
SDF | Download SDF |
Canonical SMILES | CC(=O)C1=CC(=C(C=C1)NS(=O)(=O)C)OC2=C(C=C(C=C2)F)F |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
Description | FK 3311是环氧酶(COX)-2的选择性抑制剂。 | |||||
靶点 | COX-2 | |||||
IC50 |