β-Estradiol - d3
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
雌二醇或17β-雌二醇,是一种类固醇和雌激素性激素以及主要的雌性性激素。它在发情及经期女性生殖周期的调节中非常重要,因此而命名。雌二醇对于雌性生殖组织的发育和维持是必须的,它还在许多其他组织例如骨中发挥重要作用。β-Estradiol-d3是氘代β雌二醇。
体外实验:培养基中加入β-雌二醇后剂量依赖性抑制IL-1、TNF和IL-1+ TNF诱导的IL-6的生成。Β-雌二醇也减少IL-6的mRNA水平。此外,在来源于新生鼠颅盖的原代骨细胞培养物中,雌二醇抑制TNF诱导的IL-6生成和破骨细胞发育[1]。
在体实验:已经发现,在成年雌性大鼠中,海马CA1区辐射层的突触密度对β-雌二醇的处理敏感,5天发情周期期间,卵巢类固醇的水平自然波动。几天的周期之后,突触密度恢复到发情前的水平[2]。
临床试验:与对照组的妇女相比,β-雌二醇和醋酸炔诺酮的共同使用更有效的缓解绝经后泰国妇女的更年期症状。使用12个月后,月经不调的发病率很高。但是,前3个月的治疗中,出现频率较高的异常出血。考虑到出血情况以及常见的副作用,开始治疗前应进行一定程度的咨询,以增加其治疗效果[3]。
参考文献:
[1] Girasole G, Jilka RL, Passeri G, Boswell S, Boder G, Williams DC, Manolagas SC. 17 beta-estradiol inhibits interleukin-6 production by bone marrow-derived stromal cells and osteoblasts in vitro: a potential mechanism for the antiosteoporotic effect of estrogens. J Clin Invest. 1992 Mar;89(3):883-91.
[2] Woolley CS, McEwen BS. Estradiol mediates fluctuation in hippocampal synapse density during the estrous cycle in the adult rat. J Neurosci. 1992 Jul;12(7):2549-54.
[3] Limpaphayom KK, Bunyavejchevin S. Clinical effects of 17 beta-estradiol and norethisterone acetate in postmenopausal Thai women. J Med Assoc Thai. 2000 Apr;83(4):407-16.
Physical Appearance | White solid |
Storage | Store at -20°C |
M.Wt | 275.40 |
Cas No. | 79037-37-9 |
Formula | C18H21D3O2 |
Solubility | <20.65mg/ml in ethanol; <27.54mg/ml in DMSO |
Chemical Name | (17β)-Estra-1,3,5(10)-triene-16,16,17-d3-3,17-diol |
SDF | Download SDF |
Canonical SMILES | O[C@@]1([2H])C([2H])([2H])C[C@]2([H])[C@]1(C)CC[C@@](C3=CC=C(O)C=C3CC4)([H])[C@]24[H] |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |