E3330
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
AP核酸内切酶1(APE1)是一种双功能蛋白,它包含氧化还原结构域和DNA修复域.APE1在许多实体癌中过表达,包括前列腺癌和膀胱癌\非小细胞肺癌\神经胶质瘤\髓母细胞瘤和原始神经外胚层肿瘤\骨肉瘤和生殖细胞肿瘤.E3330是抑制APE1蛋白氧化还原活性的小分子抑制剂.
体外实验:E3330显著降低人胰腺癌细胞的体外生长.在胰腺癌细胞中,通过小干扰RNA实验降低APE1表达进一步证实了这个现象.此外,缺氧可以加剧E3330的生长抑制效应,这伴随着低氧诱导因子1α的DNA结合能力的强效抑制[1].
在体实验:口服E3330减弱了血浆肿瘤坏死因子活性的升高,保护小鼠免受肝损伤.此外,体外实验中,E3330抑制脂多糖刺激的痤疮丙酸杆菌引起的小鼠腹腔巨噬细胞中肿瘤坏死因子的产生[2].
临床试验:到目前为止,E3330还处于临床前开发阶段.
参考文献：
[1] Gang-Ming Zou and Anirban Maitra. Small-molecule inhibitor of the AP endonuclease 1/REF-1 E3330 inhibits pancreatic cancer cell growth and migration. Mol Cancer Ther. 2008 Jul; 7(7): 2012–2021.
[2] Nagakawa J, Hishinuma I, Miyamoto K, Hirota K, Abe S, Yamanaka T, Katayama K, Yamatsu I. Protective effects of (2E)-3-[5-(2,3-dimethoxy-6-methyl-1,4- benzoquinoyl)]-2-nonyl-2-propenoic acid on endotoxin-mediated hepatitis in mice. J Pharmacol Exp Ther. 1992 Jul;262(1):145-50.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 378.46 |
| Cas No. | 136164-66-4 |
| Formula | C21H30O6 |
| Solubility | insoluble in H2O; ≥75.4 mg/mL in DMSO; ≥75.4 mg/mL in EtOH |
| Chemical Name | (E)-2-((4,5-dimethoxy-2-methyl-3,6-dioxocyclohexa-1,4-dien-1-yl)methylene)undecanoic acid |
| SDF | Download SDF |
| Canonical SMILES | CCCCCCCCC/C(C(O)=O)=C([H])\C(C1=O)=C(C(C(OC)=C1OC)=O)C |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
化学结构
