Digoxigenin-11-UTP
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Digoxigenin -11-UTP已被用于标记RNA探针，可以用于原位杂交及其他目的。 DIG-11-UTP是T7，SP6和T3 RNA聚合酶的底物，可以在体外转录中替代UTP，以35:65的比率进行RNA的地高辛标记。
带有T7，SP6或T3启动子的线性模板DNA分别用ATP，GTP，CTP，UTP和DIG-11-UTP与相应的RNA聚合酶进行体外转录。
标记的RNA随后可用地高辛发光检测试剂盒进行核酸*检测，或用抗DIG-AP *和CDP-Star *进行检测。
参考文献：
[1] Mugford S, et al., A Serine Carboxypeptidase-Like Acyltransferase Is Required for Synthesis of Antimicrobial Compounds and Disease Resistance in Oats, The Plant Cell 21:2473-2484 (2009)
[2]-Pontes O,et al., Natural variation in nucleolar dominance reveals the relationship between nucleolus organizer chromatin topology and rRNA gene transcription in Arabidopsis, PNAS vol. 100 no. 20 11418-11423 (2003)
[3]Schaeren-Wiemers N.et al., A single protocol to detect transcripts of various types and expression levels in neural tissue and cultured cells: in situ hybridization using digoxigenin-labelled cRNA probes, Histochemistry and Cell Biology, Volume 100, Number 6 (1993)
[4]Yanyan L. et al., PiggyBac transgenic strategies in the developing chicken spinal cord, Nucleic Acids Res., Sep 2009;10.1093/nar/gkp686
[5]Zwirglmaier K.et al., In situ functional gene analysis: recognition of individual genes by fluorescence in situ hybridization, Methods Enzymol. 397:338-51 (2005)
| Physical Appearance | Solution |
| Storage | Store at -20°C or below |
| M.Wt | 1082.92 (free acid) |
| Formula | C43H65N4O22P3 (free acid) |
| Synonyms | Digoxigenin-X-5-aminoallyl-uridine-5’-triphosphate, Triethylammonium salt, Dig-11-utp |
| Solubility | sterile clear aqueous solution, in 100 mM Tris-HCl pH 7.5 ±0.5 |
| Chemical Name | ((2R,3S,4R,5R)-5-(5-((E)-3-(6-(2-(((3S,5R,8R,9S,10S,12R,13S,14S,17R)-12,14-dihydroxy-10,13-dimethyl-17-(5-oxo-2,5-dihydrofuran-3-yl)hexadecahydro-1H-cyclopenta[a]phenanthren-3-yl)oxy)acetamido)hexanamido)prop-1-en-1-yl)-2,4-dioxo-3,4-dihydropyrimidin-1(2H |
| SDF | Download SDF |
| Canonical SMILES | O=C1OCC([C@H]2CC[C@@]3(O)[C@]2(C)[C@H](O)C[C@@]4([H])[C@@]3([H])CC[C@@]5([H])[C@]4(C)CC[C@H](OCC(NCCCCCC(NC/C=C/C6=CN([C@H]7[C@H](O)[C@H](O)[C@@H](COP(O)(OP(O)(OP(O)(O)=O)=O)=O)O7)C(NC6=O)=O)=O)=O)C5)=C1 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
