Deacetylase Inhibitor Cocktail (100× in 70% DMSO)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
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- 4. Thuy T P Nguyen, Do-Young Kim, et al. "SREBP-1c impairs ULK1 sulfhydration-mediated autophagic flux to promote hepatic steatosis in high-fat-diet-fed mice." Mol Cell. 2021 Sep 16;81(18):3820-3832.e7. PMID: 34233158
Deacetylase Inhibitor Cocktail是由几种具有协同功能的化合物混合而成,可用于维持分子的乙酰化状态。在这些靶点中,由HATs和HDACs引起的组蛋白乙酰化状态的动态变化是最常发生的,也是研究热点之一。赖氨酸乙酰化是影响蛋白质转录,DNA复制和修复,细胞周期和信号转导途径的最重要的翻译后修饰之一。乙酰转移酶和去乙酰化酶作用之间的平衡会影响染色质凝聚,转录因子活性和蛋白质聚集等,这些修饰的变化吸引了研究人员的兴趣。而我们的产品Deacetylase Inhibitor Cocktail是研究这些变化的有用工具。
Deacetylation Inhibition Cocktail的四种组分:40μM Trichostatin A, 1mM EX-527, 400 mM nicotinamide和200mM sodium butyrate。这些化合物溶于70%DMSO中。每个都有自己的特定作用。Trichostatin A可抑制class I/II HDAC。 EX-527选择性抑制SIRT1,对SIRT2和SIRT3有较弱的作用。Nicotinamide可以作为sirtuins的抑制剂(SIRT1-7,class III HDAC)。Sodium butyrate竞争性抑制class I / II HDAC。
使用前,在冰上解冻,以1:100(v / v)稀释加入溶液样品(例如细胞裂解液或组织提取液)。
应用:WB, Co-IP, pull-down, IF, IHC, Flow Cytometry, kinase assay等。
Catalog No. | Product Name | Summary | Targets | CAS Number | Smiles |
A8183 | Trichostatin A (TSA) | HDAC抑制剂 | DNA Damage/DNA Repair|HDAC | 58880-19-6 | CC(C=C(C)C=CC(=O)NO)C(=O)C1=CC=C(C=C1)N(C)C |
A4181 | EX 527 (SEN0014196) | SIRT1抑制剂 | Chromatin/Epigenetics|Sirtuin | 49843-98-3 | C1CC(C2=C(C1)C3=C(N2)C=CC(=C3)Cl)C(=O)N |
N1651 | Nicotinamide | PARP-1抑制剂 | Nature Products | 98-92-0 | C1=CC(=CN=C1)C(=O)N |
B1835 | Sodium butyrate | 组蛋白去乙酰化酶抑制剂 | 156-54-7 | CCCC(=O)[O-].[Na+] | |
-20°C条件下储存,稳定保存12个月以上。 |