DCPIB
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
DCPIB is a volume-regulated anion channel (VRAC) blocker (IC50 = 2 μM in rat pancreatic β-cells)[1], which is also a blocker of swelling-induced chloride current (ICl, swell) in various cardiovascular tissues (IC50 = 4.1 μM in CPAE cells)[2].
In intact β-cells, DCPIB inhibits glucose-stimulated insulin secretion through VSAC inhibition and indirect KATP channel activation. DCPIB reverses the shortened duration of action potential induced by cell swelling in atrial myocytes and inhibits astrocyte swelling in vitro. DCPIB (10 μM) also activates TREK1 and TRAAK K+ channels and inhibits TRESK, TASK1 and TASK3 K+ channels in vitro[3].
References:
[1]. Best L, Yates A P, Decher N, et al. Inhibition of glucose-induced electrical activity in rat pancreatic β-cells by DCPIB, a selective inhibitor of volume-sensitive anion currents. european journal of pharmacology, 2004, 489(1-2): 13-19.
[2].Decher N, et al. DCPIB is a novel selective blocker of I(Cl,swell) and prevents swelling-induced shortening of guinea-pig atrial action potential duration. Br J Pharmacol, 2001, 134(7): 1467-79.
[3]. Lv J, et al. DCPIB, an Inhibitor of Volume-Regulated Anion Channels, Distinctly Modulates K2P Channels. ACS Chem Neurosci, 2019.
Physical Appearance | White solid |
Storage | Store at -20°C |
M.Wt | 427.37 |
Cas No. | 82749-70-0 |
Formula | C22H28Cl2O4 |
Solubility | insoluble in H2O; ≥49.8 mg/mL in EtOH; ≥50.8 mg/mL in DMSO |
Chemical Name | 4-((2-butyl-6,7-dichloro-2-cyclopentyl-1-oxo-2,3-dihydro-1H-inden-5-yl)oxy)butanoic acid |
SDF | Download SDF |
Canonical SMILES | ClC(C1=C(C=C2OCCCC(O)=O)CC(CCCC)(C1=O)C3CCCC3)=C2Cl |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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