Daunorubicin HCl
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
DaunorubicinHCl是一种DNA拓扑异构酶II抑制剂[1].
Daunorubicin是一种蒽环类抗生素.它也被作为一种有效的抗肿瘤化疗药物使用,尤其是对急性髓细胞性白血病和急性淋巴细胞白血病.Daunorubicin可以影响DNA和RNA的代谢和合成.在体外试验中,daunorubicin 抑制胸腺嘧啶和尿嘧啶核苷掺入到L1210细胞中.它也能抑制标记前体掺入到从孵育细胞中分离的DNA和RNA中.当对急性淋巴细胞白血病患者中分离的白血病细胞进行测试,daunorubicin可显著抑制DNA和RNA大分子的生物合成[2,3].
参考文献:
[1] Hande K R. Etoposide: four decades of development of a topoisomerase II inhibitor. European Journal of Cancer, 1998, 34(10): 1514-1521.
[2] Momparler R L, Karon M, Siegel S E, et al. Effect of adriamycin on DNA, RNA, and protein synthesis in cell-free systems and intact cells. Cancer Research, 1976, 36(8): 2891-2895.
[3] Meriwether W D, Bachur N R. Inhibition of DNA and RNA metabolism by daunorubicin and adriamycin in L1210 mouse leukemia. Cancer research, 1972, 32(6): 1137-1142.
Storage | Store at -20°C |
M.Wt | 563.98 |
Cas No. | 23541-50-6 |
Formula | C27H30ClNO10 |
Solubility | ≥28.2 mg/mL in DMSO; insoluble in H2O; ≥2.07 mg/mL in EtOH with gentle warming and ultrasonic |
Chemical Name | (8S,10S)-8-acetyl-10-(((2R,4S,5S,6S)-4-amino-5-hydroxy-6-methyltetrahydro-2H-pyran-2-yl)oxy)-6,8,11-trihydroxy-1-methoxy-7,8,9,10-tetrahydrotetracene-5,12-dione hydrochloride |
SDF | Download SDF |
Canonical SMILES | Cl[H].O=C(C([H])([H])[H])[C@@](C([H])([H])[C@]1([H])O[C@](C([H])([H])[C@]2([H])N([H])[H])([H])O[C@](C([H])([H])[H])([H])[C@@]2([H])O[H])(C([H])([H])C3=C1C(O[H])=C(C(C4=C5C([H])=C([H])C([H])=C4OC([H])([H])[H])=O)C(C5=O)=C3O[H])O[H] |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
细胞实验 [1]: | |
细胞系 |
小鼠黑色素瘤细胞(B16F10)和小鼠成纤维细胞NIH / 3T3 |
溶解方法 |
在DMSO的溶解度>28.2mg/mL。为了获得更高的浓度,可以将离心管在37℃加热10分钟和/或在超声波浴中震荡一段时间。原液可以在-20℃以下储存几个月。 |
反应条件 |
5至500μM,24小时 |
应用 |
15μM的daunorubicin能够对黑色素瘤细胞产生毒性。与未处理的细胞相比,daunorubicin对成纤维细胞造成轻微的形态改变,并观察到细胞密度的降低,抑制细胞增殖。 |
动物实验 [2]: | |
动物模型 |
Balb-c小鼠 |
剂量 |
单次腹膜内注射,2.3至28.25μmol/ kg |
应用 |
在Balb-c小鼠中,较低剂量(1.8μmol/ kg)的daunorubicin处理组肿瘤生长抑制较轻微,而在较高剂量(7.5μmol/ kg)水平下,daunorubici处理下的肿瘤生长抑制很显著。所有来自盐水处理的动物都发生转移节点,而80%的daunorubicin处理组出现转移灶。 |
注意事项 |
请测试所有化合物在室内的溶解度,实际溶解度和理论值可能略有不同。这是由实验系统的误差引起的,属于正常现象。 |
References: [1]. Contente TC, Kretzer IF., et al. Association of daunorubicin to a lipid nanoemulsion that binds to low-density lipoprotein receptors enhances the antitumour action and decreases the toxicity of the drug in melanoma-bearing mice. J Pharm Pharmacol. 2014 Dec;66(12):1698-709. doi: 10.1111/jphp.12296 |
质量控制和MSDS
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