Dantrolene, sodium salt
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Dantrolene (sodium salt) is an antagonist of ryanodine receptor (RyR), with an IC50 of 5.9 ± 0.3 nM for RyR2 [1, 2].
RyRs are expressed on the surface of the endoplasmic reticulum and the sarcoplasmic reticulum, responsible for Ca2+ release from intracellular stores. Dysfunction of calcium homeostasis may lead to ischemia, hypoxia, seizure, trauma, anesthesia, and neurodegenerative diseases [1].
In mouse cardiomyocytes, dantrolene showed no effect on cardiac Ca2+ release in the absence of calmodulin, but reduced Ca2+ wave frequency (IC50 = 0.42 ± 0.18 µM, Emax = 47 ± 4%) and amplitude (IC50 = 0.19 ± 0.04 µM, Emax = 66 ± 4%) in the presence of 100 nM calmodulin, which implied that calmodulin was essential for dantrolene’s inhibition on RyRs [2].
In a mouse pancreatitis model induced by caerulein for 1 hour, dantrolene (5 mg/kg) reduced pancreatic trypsin activity by 59% and mitigated early ultrastructural derangements within the acinar cell. Eight hours after caerulein induction, dantrolene decreased pancreatic trypsin activity and serum amylase by 61 and 32%, respectively [3].
References:
[1]. Inan S, Wei H. The cytoprotective effects of dantrolene: a ryanodine receptor antagonist. Anesthesia & Analgesia, 2010, 111(6): 1400-1410.
[2]. Oo Y W, Gomez-Hurtado N, Walweel K, et al. Essential Role of Calmodulin in RyR Inhibition by Dantrolene. Molecular Pharmacology, 2015, 88(1): 57-63.
[3]. Orabi A I, Shah A U, Ahmad M U, et al. Dantrolene mitigates caerulein-induced pancreatitis in vivo in mice. American Journal of Physiology-Gastrointestinal and Liver Physiology, 2010, 299(1): G196-G204.
Physical Appearance | A solid |
Storage | Store at RT |
M.Wt | 336.23 |
Cas No. | 14663-23-1 |
Formula | C14H9N4NaO5 |
Solubility | insoluble in EtOH; insoluble in H2O; ≥12.2 mg/mL in DMSO |
Chemical Name | sodium (E)-1-(((5-(4-nitrophenyl)furan-2-yl)methylene)amino)-4-oxo-4,5-dihydro-1H-imidazol-2-olate |
SDF | Download SDF |
Canonical SMILES | O=C1N=C(N(C1)/N=C/C2=CC=C(C(C=C3)=CC=C3[N+]([O-])=O)O2)[O-].[Na+] |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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