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Cy7 NHS ester (non-sulfonated)
氨基反应性荧光染料,不溶于水

Cy7 NHS ester (non-sulfonated)

产品编号:A8104
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规格 单价 库存 订购数量
10mM (in 1mL DMSO) ¥4,600.00 10-15 工作日发货
5mg ¥2,100.00 现货
25mg ¥4,100.00 10-15 工作日发货

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Sample solution is provided at 25 µL, 10mM.

引用文献

1. Liang X, Li X,et al. "Nanoparticles with CD44 Targeting and ROS Triggering Properties as Effective in Vivo Antigen Delivery System." Mol Pharm. 2018 Feb 5;15(2):508-518. PMID:29323913

质量控制

化学结构

Cy7 NHS ester

相关生物数据

Cy7 NHS ester (non-sulfonated)

相关生物数据

Cy7 NHS ester (non-sulfonated)

Cy7 NHS ester (non-sulfonated) Dilution Calculator

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Cy7 NHS ester (non-sulfonated) Molarity Calculator

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化学性质

CAS号 1432019-64-1 SDF Download SDF
SMILES CC1(C)C(/C=C/C(CCC/2)=CC2=C\C=C3N(C)C4=CC=CC=C4C\3(C)C)=[N+](CCCCCC(ON(C5=O)C(CC5)=O)=O)C6=CC=CC=C61.FB(F)F.[F-]
分子式 C41H48BF4N3O4 分子量 733.64
溶解度 ≥34.1mg/mL in DMSO 储存条件 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
物理性状 A solid 运输条件 试用装:蓝冰运输。
其他可选规格:常温运输或根据您的要求用蓝冰运输。
一般建议 为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。

光谱性质

最大激发光波 (nm) 750 最大发射光波 (nm) 773
消光系数 (M-1cm-1) 199000 量子产量 0.3
CF260 0.022 CF280 0.029

产品应用

非磺化的Cyanine染料适合下列分子标记和应用:

• 可溶性蛋白,可添加有机共溶剂 • 荧光偏振 (FP)
• 抗体(在5-10%的DMSO/DMF中) • 荧光共振能量转移 (FRET)
• DNA 和 寡核苷酸 • 时间分辨荧光共振能量转移 (TR-FRET)
• 多肽 • 荧光强度(FI)
• 其他小分子  
• 有机介质中的反应(二氯甲烷,乙腈)  

产品描述

Cy7 NHS ester, also called Cy7 monofunctional N-hydroxysuccinimide ester or Cy7-NHS [1], is a near-infrared fluorescent [2] and soluble sulfonated cyanine dye [3] with a molecular weight of 828 Da[2], able to be dissolved in DMSO [1] and ready for conjugation to proteins [4]. Cy7 NHS ester acts as a donor of Cy7 [5]. Cy7 has an emission maximum at 767nm, an absorption maximum at 743nm and its ε743=200,000 cm-1M-1 [4]. Compared with Cy5.5, Cy7 has fewer sulfonate groups and an extra methine group that contributes to hydrophobicity. Antibodies labeled with either Cy7 or Cy5.5 succinimidyl esters have similar quenching efficiencies [5].

N-acylation can occur between a Cy7 NHS ester and a primary amino group. This type of reaction is also widely used to allow amide formation under milder conditions without base or coupling reagent [6]. Most derivatives of non-sulfonated cyanines have low aqueous solubility except for hydrochlorides of hydrazides and amines. For biomolecule labeling, using of organic co-solvent (5-20% of DMF or DMSO) to dissolve this molecular is necessary for efficient reaction. Cyanine dye should be dissolved in organic solvent first, and added to a solution of biomolecule (protein, peptide, amino-labeled DNA) in appropriate aqueous buffer.

Incubated with Cy7 NHS, LS174T cells showed negligible signals in fluorescent microscopic images, it illustrated that possibility of nonspecific binding between free Cy7 and LS174T cells was negligible [1].

In vivo, injection of Cy7-NHS for 15 minutes resulted in the fluorescent signal in the whole body of a mouse bearing LS174T tumors, but from the blank mouse, no autofluorescence was detected. This result suggested the rapid distribution of Cy7 NHS in the body of the mouse. Subsequently, Cy7 NHS was rapidly eliminated from the kidney and no fluorescence was detected on the mouse 96 hours post-injection [1].

References:
[1].  Peng Zou, Songbo Xu, Stephen P. Povoski, et al. Near-Infrared Fluorescence Labeled Anti-TAG-72 Monoclonal Antibodies for Tumor Imaging in Colorectal Cancer Xenograft Mice. Mol Pharm., 2009, 6(2): 428-440.
[2].  Martin L. Brady, Raghu Raghavan, Deep Singh, et al. In vivo performance of a microfabricated catheter for intraparenchymal delivery. Journal of Neuroscience Methods, 2014, 229: 76-83.
[3].  Valeri Metelev, Surong Zhang, David Tabatadze, et al. The three-dimensional context of a double helix determines fluorescence of the internucleoside-tethered pair of fluorophores. Mol Biosyst., 2013, 9(10): 2447-2453.
[4].  Manfred Birchler, Giovanni Neri, Lorenzo Tarli, et al. Infrared photodetection for the in vivo localisation of phage-derived antibodies directed against angiogenic markers. Journal of Immunological Methods, 1999, 231: 239-248.
[5].  Benedict Law, Alejandro Curino, Thomas H. Bugge, et al. Design, Synthesis, and Characterization of Urokinase Plasminogen-Activator-Sensitive Near-Infrared Reporter. Chemistry & Biology, 2004, 11: 99-106.
[6].  M. Bai and D.J. Bornhop. Recent Advances in Receptor-Targeted Fluorescent Probes for In Vivo Cancer Imaging. Current Medicinal Chemistry, 2012, 19(1): 1-17.