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Cy7 maleimide (non-sulfonated)
巯基反应性荧光染料,不溶于水

Cy7 maleimide (non-sulfonated)

产品编号:A8141
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规格 单价 库存 订购数量
5mg ¥2,100.00 现货
25mg ¥4,100.00 10-15 工作日发货

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Sample solution is provided at 25 µL, 10mM.

质量控制

化学结构


Cy7 maleimide (non-sulfonated)

相关生物数据

Cy7 maleimide (non-sulfonated)

Cy7 maleimide (non-sulfonated) Dilution Calculator

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Cy7 maleimide (non-sulfonated) Molarity Calculator

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化学性质

CAS号 SDF Download SDF
化学名 6-[(2E)-3,3-dimethyl-2-[(2E)-2-[3-[(E)-2-(1,3,3-trimethylindol-1-ium-2-yl)ethenyl]cyclohex-2-en-1-ylidene]ethylidene]indol-1-yl]-N-[2-(2,5-dioxopyrrol-1-yl)ethyl]hexanamide
SMILES CC1(C2=CC=CC=C2[N+](=C1C=CC3=CC(=CC=C4C(C5=CC=CC=C5N4CCCCCC(=O)NCCN6C(=O)C=CC6=O)(C)C)CCC3)C)C
分子式 C43H51ClN4O3 分子量 707.34
溶解度 soluble in organic solvents (DMSO, DMF, dichloromethane), low solubility in water 储存条件 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
运输条件 试用装:蓝冰运输。
其他可选规格:常温运输或根据您的要求用蓝冰运输。
一般建议 为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。

光谱性质

最大激发光波 (nm) 750 最大发射光波 (nm) 773
消光系数 (M-1cm-1) 199000 量子产量 0.3
CF260 0.022 CF280 0.029

产品应用

非磺化的Cyanine染料适合下列分子标记和应用:

• 可溶性蛋白,可添加有机共溶剂 • 荧光偏振 (FP)
• 抗体(在5-10%的DMSO/DMF中) • 荧光共振能量转移 (FRET)
• DNA 和 寡核苷酸 • 时间分辨荧光共振能量转移 (TR-FRET)
• 多肽 • 荧光强度(FI)
• 其他小分子  
• 有机介质中的反应(二氯甲烷,乙腈)  

产品描述

Cy7 maleimide is a near-infrared, sulfhydryl reactive dye which has low aqueous solubility. This reagent can label the proteins contain free sulfhydryl groups. Labeled proteins thus obtained are used in NIR bioimaging applications. In tissues or in live organism, NIR imaging systems can be used to visualize distribution of labeled proteins. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer.

In splenocytes, for ease of detection, P22 particles were labeled internally with Cy7-maleimide. P22-Cy7 alone or P22-Cy7 decorated with DecSelf was incubated with splenocytes at 37℃ to encourage phagocytosis [1].

Reference:
[1].  Schwarz, B.; Madden, P.; Avera, J.; Gordon, B.; Larson, K.; Miettinen, H.; Uchida, M.; LaFrance, B.; Basu, G.; Rynda-Apple, A.; Douglas, T. Symmetry Controlled, Genetic Presentation of Bio-Active Proteins on the P22 Virus-Like Particle Using an External Decoration Protein. ACS Nano, 2015, 9(9), 9134–9147.