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Cy5 amine (non-sulfonated)
可以与NHS酯,羧基(碳二亚胺活化后)和环氧化物等反应性基团反应

Cy5 amine (non-sulfonated)

产品编号:A8143
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规格 单价 库存 订购数量
5mg ¥2,100.00 现货
25mg ¥4,100.00 现货

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Sample solution is provided at 25 µL, 10mM.

引用文献

1. Qin Jiang, Zimiao Luo, et al. "Red blood cell membrane-camouflaged melanin nanoparticles for enhanced photothermal therapy." Biomaterials. S0142-9612(17)30483-0.

质量控制

化学结构

Cy5 amine (non-sulfonated)

相关生物数据

Cy5 amine (non-sulfonated)

相关生物数据

Cy5 amine (non-sulfonated)

Cy5 amine (non-sulfonated) Dilution Calculator

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Cy5 amine (non-sulfonated) Molarity Calculator

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化学性质

CAS号 SDF Download SDF
化学名 6-[6-[(2E)-3,3-dimethyl-2-[(2E,4E)-5-(1,3,3-trimethylindol-1-ium-2-yl)penta-2,4-dienylidene]indol-1-yl]hexanoylamino]hexylazanium
SMILES CC1(C2=CC=CC=C2[N+](=C1C=CC=CC=C3C(C4=CC=CC=C4N3CCCCCC(=O)NCCCCCC[NH3+])(C)C)C)C
分子式 C38H54Cl2N4O 分子量 653.77
溶解度 ≥48mg/mL in DMSO 储存条件 Store at -20°C
物理性状 A solid 运输条件 试用装:蓝冰运输。
其他可选规格:常温运输或根据您的要求用蓝冰运输。
一般建议 为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。

光谱性质

最大激发光波 (nm) 646 最大发射光波 (nm) 662
消光系数 (M-1cm-1) 250000 量子产量 0.2
CF260 0.03 CF280 0.04

产品应用

非磺化的Cyanine染料适合下列分子标记和应用:

• 可溶性蛋白,可添加有机共溶剂 • 荧光偏振 (FP)
• 抗体(在5-10%的DMSO/DMF中) • 荧光共振能量转移 (FRET)
• DNA 和 寡核苷酸 • 时间分辨荧光共振能量转移 (TR-FRET)
• 多肽 • 荧光强度(FI)
• 其他小分子  
• 有机介质中的反应(二氯甲烷,乙腈)  

产品描述

Cy5 amine is a reactive dye which contains amino group. This dye can be coupled with a variety of activated esters and other electrophilic reagents. For instance, this amine can be coupled with EDC-activated carboxylic groups. With this bright and photostable reagent, many different methods of fluorescence detection is available and colorful fluorophore can also be easily detected in small quantities by naked human eye. For biomolecule labeling, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer.

In A2780 cells, the conjugates polymer 2P-Cy3-Cy5 can be used for the analysis of the pair-correlation functions between Cy3 and Cy5. Treated with 2P-Cy3-Cy5 for several hours show similar shape and characteristic decay lengths [1].

Reference:
[1]Hartley, J. M.; Zhang, R.; Gudheti, M.; Yang, J.; Kopeek, J. Tracking and quantifying polymer therapeutic distribution on a cellular level using 3D dSTORM. Journal of Controlled Release, 2016, 231, 50–59.