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Cy5.5 NHS ester (non-sulfonated)

现货
Catalog No.
A8103
氨基反应性荧光染料,不溶于水
组合的产品项目
规格价格库存 数量
5mg
¥ 2,100.00
现货
25mg
¥ 4,100.00
现货

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Background

Cyanine5.5 NHS ester is a reactive dye for the labeling of peptides, proteins, and oligonucleotides that contain amino-groups. Cy5.5-NHS ester is a near-infrared fluorescence dye able to dissolve in DMF and DMSO with the excitation maximum and emission maximum around 675 nm and 693 nm respectively [1]. The reagent is not only used for labeling moleculars contain amino groups but also used for labeling plasmid DNA [2].For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer.

Through the optical imaging of subcutaneous tumor xenografted mice, the subcutaneous U87MG tumor could be clearly distinguished from the surrounding background tissue from 30 min up to 24 h postinjection of 0.5 nmol Cy5.5-NHS. The tumor uptake of Cy5.5-NHS reached a maximum 30 minutes postinjection and slowly washed out over time [3].

References:
[1].  Ju Hee Ryu, Jin Hee Na, Ho Kyung Ko, et al. Non-invasive optical imaging of cathepsin B with activatable fluorogenic nanoprobes in various metastatic models. Biomaterials, 2014, 35: 2302-2311.
[2].  Laura-Lee Farrell, Joel Pepin, Cezary Kucharski, et al. A comparison of the effectiveness of cationic polymers poly-L-lysine (PLL) and polyethylenimine (PEI) for non-viral delivery of plasmid DNA to bone marrow stromal cells (BMSC). European Journal of Pharmaceutics and Biopharmaceutics, 2007, 65: 388–397.
[3].  Zhen Cheng, Jelena Levi, Zhengming Xiong, et al. Near-Infrared Fluorescent Deoxyglucose Analog for Tumor Optical Imaging in Cell Culture and in Living Mice. Bioconjug Chem., 2006, 17(3):662-669.

文献引用

1. Zou S, Duan B, et al. "Inhibition of tumor growth by β-glucans through promoting CD4(+) T cell immunomodulation and neutrophil-killing in mice." Carbohydr Polym.2019 Jun 1;213:370-381. PMID:30879681
2. Cheng M, Zhang Y, et al. "One-pot synthesis of acid-induced in situ aggregating theranostic gold nanoparticles with enhanced retention in tumor cells. " Biomater Sci. 2019 Mar 6. PMID:30839987
3. Jiang C, Qi Z, et al. "ATP-responsive low-molecular-weight polyethylenimine-based supramolecular assembly via host-guest interaction for gene delivery." Biomacromolecules. 2018 Dec 5. PMID:30516950
4. Lu J, Zhao Y, et al."Biofunctional polymer-lipid hybrid high density lipoprotein-mimicking nanoparticles loading anti-miR155 for combined antiatherogenic effects on macrophages." Biomacromolecules. 2017 Aug 3. PMID:28738148

Chemical Properties

Physical AppearanceA solid
Storage24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
该产品在溶液中不稳定,建议现配现用
M.Wt716.31
FormulaC44H46ClN3O4
Solubility≥35.8mg/mL in DMSO
Chemical Name3-(6-((2,5-dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)-1,1-dimethyl-2-((1E,3E,5E)-5-(1,1,3-trimethyl-1H-benzo[e]indol-2(3H)-ylidene)penta-1,3-dien-1-yl)-1H-benzo[e]indol-3-ium
SDFDownload SDF
Canonical SMILESCC1(C)C2=C3C(C=CC=C3)=CC=C2[N+](CCCCCC(ON4C(CCC4=O)=O)=O)=C1/C=C/C=C/C=C5C(C)(C)C6=C(C=CC=C7)C7=CC=C6N\5C
运输条件试用装:蓝冰运输。 其他可选规格:常温运输或根据您的要求用蓝冰运输。
一般建议为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。

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化学结构

Cy5.5 NHS ester

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Cy3.5 NHS ester (non-sulfonated)

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Cy3.5 NHS ester (non-sulfonated)

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Cy3.5 NHS ester (non-sulfonated)

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Cy3.5 NHS ester (non-sulfonated)

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Cy3.5 NHS ester (non-sulfonated)