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Cy3 NHS ester (non-sulfonated)

现货
Catalog No.
A8100
氨基反应性荧光染料,不溶于水
组合的产品项目
规格价格库存 数量
10mM (in 1mL DMSO)
¥ 4,000.00
现货
5mg
¥ 2,100.00
现货
25mg
¥ 4,100.00
Ship with 10-15 days

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Background

Cyanine, a kind of synthetic dye belonging to the polymethine group, is still used in industry to increase the sensitivity range of photographic emulsions (such as increasing the wavelength range and making the film panchromatic), and in biotechnology more recently. As fluorescent dyes, cyanines have been particularly used in biomedical imaging. Depending on their structure, cyanines can cover the spectrum from UV to IR.

Cy3 NHS ester, a reactive dye for labeling of amino-groups in biomolecules, is a Cy3® NHS ester analog. This reagent is commonly used for the labeling of soluble proteins, peptides, and oligonucleotides or DNA. Cy3 NHS ester requires the use of organic co-solvent (such as DMF, or DMSO) for the labeling, however, considering the usage of water-soluble sulfo-Cy3 NHS ester for delicate proteins, such co-solvent is not necessary. Cy3 fluoresce is in the orange region (~550 nm excitation, ~570 nm emission), while Cy5 is fluorescent in the red (~650/670 nm) but absorbs in the orange (~649 nm). Cy3 can be monitored by fluorometers, imagers, and microscopes with standard Tetramethylrhodamine (TRITC) filters. Due to its inherently high extinction coefficient, Cy3 is even detected by naked eye on gels and in solution easily.

Reference:
http://en. wikipedia.org/wiki/Cyanine

文献引用

1. Hassnain Asgar, Liyan Jacob ,et al. "Fast spontaneous emission and high Förster resonance energy transfer rate in hybrid organic/inorganic plasmonic nanostructures." arXiv preprint arXiv:1804.09637, 2018.

Chemical Properties

Physical AppearanceA solid
Storage24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
M.Wt590.15
FormulaC34H40ClN3O4
Solubility≥59mg/mL in DMSO, ≥25.3 mg/mL in EtOH with ultrasonic, <3.18 mg/mL in H2O
Chemical Name(2,5-dioxopyrrolidin-1-yl) 6-[(2E)-3,3-dimethyl-2-[(E)-3-(1,3,3-trimethylindol-1-ium-2-yl)prop-2-enylidene]indol-1-yl]hexanoate
SDFDownload SDF
Canonical SMILESCC1(C2=CC=CC=C2[N+](=C1C=CC=C3C(C4=CC=CC=C4N3CCCCCC(=O)ON5C(=O)CCC5=O)(C)C)C)C
运输条件试用装:蓝冰运输。 其他可选规格:常温运输或根据您的要求用蓝冰运输。
一般建议为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。

质量控制

化学结构

Cy3 NHS ester (non-sulfonated)

相关生物数据

CORM-3