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Cy3 maleimide (non-sulfonated)
巯基反应性荧光染料,不溶于水

Cy3 maleimide (non-sulfonated)

产品编号:A8138
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规格 单价 库存 订购数量
5mg ¥2,100.00 现货
25mg ¥4,100.00 现货

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Sample solution is provided at 25 µL, 10mM.

质量控制

化学结构

Cy3 maleimide (non-sulfonated)

相关生物数据

Cy3 maleimide (non-sulfonated)

Cy3 maleimide (non-sulfonated) Dilution Calculator

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Cy3 maleimide (non-sulfonated) Molarity Calculator

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化学性质

CAS号 SDF Download SDF
化学名 1-(6-((2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl)amino)-6-oxohexyl)-3,3-dimethyl-2-((1E,3E)-3-(1,3,3-trimethylindolin-2-ylidene)prop-1-en-1-yl)-3H-indol-1-ium
SMILES CC1(C)C2=CC=CC=C2[N+](CCCCCC(NCCN3C(C=CC3=O)=O)=O)=C1/C=C/C=C4C(C)(C)C5=CC=CC=C5N\4C
分子式 C36H43ClN4O3 分子量 615.20
溶解度 soluble in organic solvents (DMF, DMSO, dichloromethane), insoluble in water 储存条件 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate
运输条件 试用装:蓝冰运输。
其他可选规格:常温运输或根据您的要求用蓝冰运输。
一般建议 为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。

光谱性质

最大激发光波 (nm) 555 最大发射光波 (nm) 570
消光系数 (M-1cm-1) 150000 量子产量 0.31
CF260 0.04 CF280 0.09

产品应用

非磺化的Cyanine染料适合下列分子标记和应用:

• 可溶性蛋白,可添加有机共溶剂 • 荧光偏振 (FP)
• 抗体(在5-10%的DMSO/DMF中) • 荧光共振能量转移 (FRET)
• DNA 和 寡核苷酸 • 时间分辨荧光共振能量转移 (TR-FRET)
• 多肽 • 荧光强度(FI)
• 其他小分子  
• 有机介质中的反应(二氯甲烷,乙腈)  

产品描述

Cy3 maleimide is a selective and efficient fluorophore dye with low aqueous solubility. This labeling reagent can be used to attach Cy3 fluorophore to proteins and peptides which contain cysteine residues, as well as to other thiolate molecules (such as thiol-containing oligonucleotides). Before the labeling reactions, Cystines should be reduced with TCEP (tris-carboxyethylphosphine). For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer.

MSNs were labeled by Cy3 maleimide named MSN-Cy3s at the concentration of 10 μg ml-1. MSN-Cy3s incubated with MEF-mEGFP cells on a glass coverslip in culture medium for 2 h To further visualize the MSN locations in the cytosol we used super-resolution microscopy (3D SIM). The visually microscope image results confirm that MSNs were internalized by MEF-mEGFP cells [1].

Reference:
[1].  Chiu, H.-Y.; Deng, W.; Engelke, H.; Helma, J.; Leonhardt, H.; Bein, T. Intracellular chromobody delivery by mesoporous silica nanoparticles for antigen targeting and visualization in real time. Scientific Reports, 2016, 6, 25019.