Cy3 hydrazide (non-sulfonated)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Cy3 hydrazide是一种羰基反应性染料,是Cy3® hydrazide的类似物。
Cyanine3染料可用于标记生物学分子,用于荧光成像和其它的基于荧光的生化分析。他们被广泛用于标记多肽、蛋白和寡核苷酸等。
Cy3 hydrazide可用于标记不同的含羰基的分子,如碘酸氧化后的抗体和其它糖蛋白,经历脱氨或氧化应激的蛋白,或还原糖。Cyanine3跟许多荧光设备都是兼容的。
参考文献:
Pan D, Qin H, Cooperman BS. Synthesis and functional activity of tRNAs labeled with fluorescent hydrazides in the D-loop. RNA. 2009 Feb;15(2):346-54.
Tamarit J, de Hoogh A, Obis E, et al. Analysis of oxidative stress-induced protein carbonylation using fluorescent hydrazides. J Proteomics. 2012 Jun 27;75(12):3778-88.
Kameyama A, Kaneda Y, Yamanaka H, et al. Detection of oligosaccharides labeled with cyanine dyes using matrix-assisted laser desorption/ionization mass spectrometry. Anal Chem. 2004 Aug 1;76(15):4537-42.
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 543.57 |
Formula | C30H40Cl2N4O |
Solubility | ≥54.4 mg/mL in DMSO; ≥48.2 mg/mL in EtOH; ≥50.8 mg/mL in H2O |
Chemical Name | 1-(6-hydrazinyl-6-oxohexyl)-3,3-dimethyl-2-((1E,3E)-3-(1,3,3-trimethylindolin-2-ylidene)prop-1-en-1-yl)-3H-indol-1-ium chloride hydrochloride |
SDF | Download SDF |
Canonical SMILES | CN1C2=CC=CC=C2C(C)(C)/C1=C\C=C\C3=[N+](CCCCCC(NN)=O)C4=CC=CC=C4C3(C)C.[Cl-].Cl |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
最大激发光波 (nm) | 555 | 最大发射光波 (nm) | 570 |
消光系数 (M-1cm-1) | 150000 | 量子产量 | 0.31 |
CF260 | 0.04 | CF280 | 0.09 |
质量控制和MSDS
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