Curcumin
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Curcumin是酪氨酸酶的抑制剂，IC50值为47 μM [1]。
Curcumin是具有抗癌功效的天然化合物。目前正在进行其对癌症预防效果的临床研究。这种抗癌功能有生化机制参与。Curcumin参与多种信号途径，包括转录因子、生长因子、炎性细胞因子、受体和酶。 I期临床试验测试了curcumin的毒性和耐受性，使用剂量为12 g/day的 curcumin没有毒性。Curcumin的生物利用度较差。在I / II期临床试验中，口服利用率约为1％，这阻碍了curcumin的临床应用[2]。
有研究表明curcumin降低了SH-SY5Y神经母细胞瘤细胞中Aβ40和Aβ42的产生，下调细胞中PS1和GSK-3β的表达，最终抑制Aβ的形成，在AD 病人中，curcumin是一种潜在的治疗剂[3]。
参考文献：
[1] Sachiko Shirota, Kouji Miyazaki, Ritsuo Aiyama, Minoru Ichioka and Teruo Yokokura. Tyrosinase inhibitors from crude drugs. Biol. Pharm. Bull. 1994, 17 (2): 266-269.
[2] Wungki Park, A.R.M Ruhul Amin, Zhuo Georgia Chen, and Dong M. Shin. New perspectives of curcumin in cancer prevention. Cancer Prev Res (Phila). 2013, 6(5): 387–400.
[3] Zhang Xiong, Zhang Hongmei, SiLu, LiYu. Curcumin mediates presenilin-1 activity to reduce β-amyloid production in a model of Alzheimer’s disease. Pharmacological Reports. 2013, 63: 1101-1108.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 368.39 |
| Cas No. | 458-37-7 |
| Formula | C21H20O6 |
| Synonyms | Indian Saffron;Turmeric yellow;Natural Yellow 3;Diferuloylmethane |
| Solubility | ≥36.8 mg/mL in DMSO; insoluble in H2O; ≥3.5 mg/mL in EtOH with gentle warming and ultrasonic |
| Chemical Name | (1E,6E)-1,7-bis(4-hydroxy-3-methoxyphenyl)hepta-1,6-diene-3,5-dione |
| SDF | Download SDF |
| Canonical SMILES | O=C(CC(/C=C/C1=CC=C(O)C(OC)=C1)=O)/C=C/C2=CC=C(O)C(OC)=C2 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| Cell experiment:[1] | |
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Cell lines |
B16-R melanoma cells resistant to doxorubicin |
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Reaction Conditions |
1 ~ 200 μM curcumin for 24, 36 or 48 h incubation |
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Applications |
Curcumin was found to be cytotoxic in vitro for B16-R melanoma cells resistant to doxorubicin either cultivated as monolayers (1 ~ 100 μM) or grown in three-dimensional cultures (1 ~ 200 μM). The cytotoxic effect observed in the 2 culture types was related to the induction of programmed cell death. |
| Animal experiment:[1] | |
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Animal models |
Female B6D2F1 mice (6 ~ 8 weeks old) challenged subcutaneously with B16-R melanoma cells |
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Dosage form |
25 mg/kg Once daily by intraperitoneal injection |
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Applications |
The combination treatment consisting of curcumin and soluble B16-R proteins resulted in substantial inhibition of growth of B16-R melanoma, whereas each treatment by itself showed little effect. Moreover, animals receiving the combination therapy exhibited an enhancement of their humoral anti-soluble B16-R protein immune response and a significant increase in their median survival time. Therefore, curcumin may provide a valuable tool for the development of a therapeutic combination against the melanoma. |
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Note |
The technical data provided above is for reference only. |
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References: 1. Odot J, Albert P, Carlier A, et al. In vitro and in vivo anti-tumoral effect of curcumin against melanoma cells. International Journal of Cancer, 2004, 111(3): 381-387. |
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质量控制和MSDS
- 批次:
化学结构
