Clofarabine
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Clofarabine是DNA合成抑制剂,也是脱氧胞苷激酶(dCK)的底物。
DCK是DNA合成salvage途径中的一个关键的胞质酶,可负责Clofarabine的磷酸化。
Clofarabine被磷酸化形成Clofarabine三磷酸,与dATP竞争DNA聚合酶-α和-ε。同时,Clofarabine-单磷酸盐被并入内部和终端的DNA位点上,它可削减DNA的延长并修复。Clofarabine三磷酸抑制核苷酸还原酶的IC50值65 nM。Clofarabine可减少dCTP和dATP量。通过核苷转运蛋白hENT1、hENT2和hCNT2高效地运输Clofarabine到细胞里。Clofarabine导致细胞色素c、细胞凋亡蛋白酶激活因子1(APAF1)、细胞凋亡诱导因子(AIF)和Caspase-9释放而进入胞液。在快速增长和静止的肿瘤中,由于Clofarabine抑制DNA合成并诱导细胞凋亡而具有抗癌活性。
植入人肿瘤的无胸腺裸体或严重联合免疫缺陷的小鼠,Clofarabine接种到小鼠腹腔里有显著的抗肿瘤活性。
参考文献:
[1]. Bonate PL, Arthaud L, Cantrell WR Jr, et al. Discovery and development of clofarabine: a nucleoside analogue for treating cancer. Nat Rev Drug Discov, 2006, 5(10): 855-863.
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 303.68 |
Cas No. | 123318-82-1 |
Formula | C10H11ClFN5O3 |
Solubility | insoluble in H2O; insoluble in EtOH; ≥15.18 mg/mL in DMSO |
Chemical Name | (2R,3R,4S,5R)-5-(6-amino-2-chloropurin-9-yl)-4-fluoro-2-(hydroxymethyl)oxolan-3-ol |
SDF | Download SDF |
Canonical SMILES | C1=NC2=C(N1C3C(C(C(O3)CO)O)F)N=C(N=C2N)Cl |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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