CK-636
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
CK-636是Arp2/3复合体的抑制剂，对人、裂殖酵母和牛Arp2/3复合体诱导的肌动蛋白聚合的IC50为4 μM、24 μM和32 μM[1]。
Arp2/3复合体可能参与形态发生和细胞粘附，对表皮功能是必需的，它调节紧密连接和终端分化，促进表皮屏障形成[2]。
CK-636在Arp2和Arp3之间结合，阻止Arp2和Arp3进入活性构象，抑制Arp2/3复合体形成核肌动蛋白丝的能力。使用Listeria运动实验，以确定CK-636是否能抑制活细胞中Arp2/3复合体介导的肌动蛋白聚合。Listeria实验表明，在感染的SKOV3细胞中，CK-636以浓度依赖性方式减少肌动蛋白丝的形成， IC50值为22 μM[1]。
参考文献：
[1]. Nolen BJ, Tomasevic N, Russell A, et al. Characterization of two classes of small molecule inhibitors of Arp2/3 complex. Nature, 2009, 460(7258): 1031-1034.
[2]. Zhou K, Muroyama A, Underwood J, et al. Actin-related protein2/3 complex regulates tight junctions and terminal differentiation to promote epidermal barrier formation. Proc Natl Acad Sci, 2013, 110(40): E3820-E3829.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 284.38 |
| Cas No. | 442632-72-6 |
| Formula | C16H16N2OS |
| Solubility | ≥10.8 mg/mL in DMSO; insoluble in H2O; ≥2.67 mg/mL in EtOH with gentle warming and ultrasonic |
| Chemical Name | N-[2-(2-methyl-1H-indol-3-yl)ethyl]thiophene-2-carboxamide |
| SDF | Download SDF |
| Canonical SMILES | CC1=C(C2=CC=CC=C2N1)CCNC(=O)C3=CC=CS3 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
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