Chlorpromazine HCl
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
多巴胺受体是一类G蛋白偶联受体,在中枢神经系统中有突出地位.多巴胺受体参与多种神经过程.因此,多巴胺受体是常见的神经药物靶点.抗精神病药经常是多巴胺受体拮抗剂,而典型的神经兴奋剂则通常是多巴胺受体的间接激动剂.Chlorpromazine是一种多巴胺拮抗剂.
在体外:chlorpromazine的抗精神病活性与其拮抗多巴胺受体的功能有关,其具有吩噻嗪环结构,可与多巴胺受体相互作用.此外,chlorpromazine可抑制[3H]螺哌隆的结合,其抑制曲线与单一位点结合的类型相一致[1].
在体内:大鼠每天使用chlorpromazine持续21日可导致僵住症\对僵住症的耐受和PCP挑战带来的自主活动敏化.结果表明每日chlorpromazine治疗可诱导DA/NMDA受体对PCP挑战带来的总的自主活动敏感[2].
临床试验:Chlorpromazine在临床上是一种传统的抗精神病药,自从1954年被FDA批准后一直用于处理精神分裂.
参考文献：
[1] Harrold MW, Chang YA, Wallace RA, Farooqui T, Wallace LJ, Uretsky N, Miller DD. Charged analogues of chlorpromazine as dopamine antagonists. J Med Chem. 1987 Sep;30(9):1631-5.
[2] Nsimba SE. Effects of daily chlorpromazine administration on behavioural and physiological parameters in the rat. Indian J Physiol Pharmacol. 2009 Jul-Sep;53(3):209-18.
- 1. Jun Wang, Wei Tang, et al. "Inflammatory tumor microenvironment responsive neutrophil exosomes-based drug delivery system for targeted glioma therapy." Biomaterials. 2021 Jun;273:120784. PMID:33848731
- 2. Dong Y, Gao J, et al. "Antigen-Conjugated Silica Solid Sphere as Nanovaccine for Cancer Immunotherapy." Int J Nanomedicine. 2020;15:2685-2697. PMID:32368049
- 3. Zhou J, Ma S, et al. "Virus-Inspired Mimics: Dual-pH-Responsive Modular Nanoplatforms for Programmable Gene Delivery without DNA Damage with the Assistance of Light." ACS Appl Mater Interfaces. 2020;12(20):22519-22533. PMID:32329598
- 4. Wei P, Ning M, et al. "Spiroplasma eriocheiris entered Drosophila Schneider 2 cells and relied on clathrin-mediated endocytosis and macropinocytosis." Infect Immun. 2019 Aug 26. pii: IAI.00233-19. PMID:31451616
| Storage | Store at -20°C |
| M.Wt | 355.33 |
| Cas No. | 69-09-0 |
| Formula | C17H19ClN2S·HCl |
| Solubility | ≥17.77 mg/mL in DMSO; ≥71.4 mg/mL in H2O; ≥74.8 mg/mL in EtOH |
| Chemical Name | 3-(2-chlorophenothiazin-10-yl)-N,N-dimethylpropan-1-amine;hydrochloride |
| SDF | Download SDF |
| Canonical SMILES | CN(C)CCCN1C2=CC=CC=C2SC3=C1C=C(C=C3)Cl.Cl |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| 细胞实验 [1]: | |
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细胞系 |
海马神经元 |
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制备方法 |
在DMSO中的溶解度大于10 mM。若配制更高浓度的溶液，一般步骤如下：请将试管置于37 °C加热10分钟和/或将其置于超声波浴中震荡一段时间。原液于-20 °C可放置数月。 |
|
反应条件 |
10 ~ 100 μM |
|
实验结果 |
10 ~ 100 μM的Chlorpromazine HCl呈剂量依赖性地降低mIPSC幅度。此外，当浓度≥ 30 μM时，Chlorpromazine HCl呈剂量依赖性地显著加快mIPSC衰减时间。然而，对照组与Chlorpromazine HCl治疗组的10 ~ 90%上升时间无显著差异。 |
| 动物实验 [2]: | |
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动物模型 |
大鼠缺氧模型 |
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给药剂量 |
30 mg/kg；腹腔注射 |
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实验结果 |
在缺氧大鼠模型中，Chlorpromazine HCl减少脑组织突触传递的不可逆缺失。Chlorpromazine HCl还可以减缓Ca2+流入神经元，从而显著延迟缺氧诱导扩散抑制的发生。 |
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注意事项 |
请于室内测试所有化合物的溶解度。虽然化合物的实际溶解度可能与其理论值略有不同，但仍处于实验系统误差的允许范围内。 |
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References: [1]. Mozrzymas JW, Barberis A, Michalak K, Cherubini E. Chlorpromazine inhibits miniature GABAergic currents by reducing the binding and by increasing the unbinding rate of GABAA receptors. J Neurosci. 1999 Apr 1;19(7):2474-88. [2]. Balestrino M, Somjen GG. Chlorpromazine protects brain tissue in hypoxia by delaying spreading depression-mediated calcium influx. Brain Res. 1986 Oct 22;385(2):219-26. |
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质量控制和MSDS
- 批次:
化学结构
相关生物数据
