CH 223191
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
CH 223191 is a potent aryl hydrocarbon receptor (AhR) antagonist with an IC50 value of 30 nM [1].
The aryl hydrocarbon receptor (AhR) is a cytosolic ligand-activated basic helix-loop-helix transcription factor. The toxicities of 2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin (TCDD) are mostly mediated by binding and activating AhR, which causes nuclear signal transduction and adverse effects.
CH 223191 inhibits TCDD-induced AhR-dependent transcription in vitro. By the HepG2-DER-luc-cell-based assay, CH 223191 showed the most potent inhibitory (IC50=0.03±0.005 mΜ). CH 223191 had no agonist-like effect (at concentrations up to 100 μM) compared with known AhR antagonists such as resveratrol and α-naphthoflavone. Besides, CH 223191 had no effect on estrogen receptor-mediated luciferase activity while flavone potentiated that in MCF-7 breast cancer cells [1].
Male mice were administrated with vehicle or CH 223191 (10mg/kg) orally once a day for 25 days and treated with TCDD (100μg/kg) once after the first week of CH 223191 administration. Expression of cytochrome P450 1A1protein from extracts of the liver was quantitated, which showed significant suppression by treatment of CH 223191 (10 mg/kg). By measuring plasma AST and ALT levels, the level of TCDD-induced toxicity was decreased apparently by CH 223191. By measuring body weights, the drug significantly avoided the symptoms of severe wasting caused by TCDD [1].
Reference:
[1]. Kim S-H, Henry E C, Kim D-Y, et al. Novel Compound 2-Methyl-2H-pyrazole-3-carboxylic Acid (2-methyl-4-o-tolylazo-phenyl)-amide (CH-223191) Prevents 2,3,7,8-TCDD-Induced Toxicity by Antagonizing the Aryl Hydrocarbon Receptor. Molecular pharmacology, 2006, 69(6):1871-1878.
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 333.39 |
Cas No. | 301326-22-7 |
Formula | C19H19N5O |
Solubility | ≥33.3 mg/mL in DMSO; insoluble in H2O; ≥2.31 mg/mL in EtOH |
Chemical Name | 2-methyl-N-[2-methyl-4-[(2-methylphenyl)diazenyl]phenyl]pyrazole-3-carboxamide |
SDF | Download SDF |
Canonical SMILES | CC1=CC=CC=C1N=NC2=CC(=C(C=C2)NC(=O)C3=CC=NN3C)C |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
- 批次: