CD 2665
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
CD 2665是视黄酸受体β(RARβ)/RARγ拮抗剂[1][2][3],对RARβ和RARγ的结合Kd值分别为306 nM和110 nM[3]。
视黄酸受体包括RARα、RARβ和RARγ。这类物质可结合视黄酸(RA)的9-顺式和全反式立体异构体[1],并可作为转录因子介导RA信号[2]。
在HiB5细胞中,CD 2665(1 μM)不能拮抗9-顺式和全反式RA诱导的荧光素酶活性,但在3XbRARE-Luc转染细胞中,可部分阻断CD 666(一种RARγ选择性激动剂,100 nM)诱导的荧光素酶活性。CD 2665在1 μM时可降低活细胞数至对照处理细胞60%的水平。CD 2665(1 μM)可明显地拮抗CD 666(100 nM)处理引起的细胞数上升[4]。
视黄酸的生物合成也可被习惯性乙醇消费扰乱。使用CD 2665不会改变酒精处理小鼠的血液酒精水平,但能完全逆转酒精摄入导致的自发交替率下降。在酒精处理小鼠中,使用CD2665达22天可显著减少tTG(一种视黄酸的靶基因)的mRNA表达和酶活,并能使表达水平正常化至对照组小鼠的水平[2]。
参考文献:
[1]. Yasmin Marikar, ZengQuan Wang, Elizabeth A. Duell, et al. Retinoic Acid Receptors Regulate Expression of Retinoic Acid 4-Hydroxylase that Specifically Inactivates All-Trans Retinoic Acid in Human Keratinocyte HaCaT Cells. The Journal of Investigative Dermatology, 1998, 111(3):434-439.
[2]. Serge Alfos, Catherine Boucheron, Véronique Pallet, et al. A Retinoic Acid Receptor Antagonist Suppresses Brain Retinoic Acid Receptor Overexpression and Reverses a Working Memory Deficit Induced by Chronic Ethanol Consumption in Mice. Alcohol Clin Exp Res., 2001, 25(10): 1506-1514.
[3]. Zsuzsa Szondy, Uwe Reichert, Jean-Michel Bernardon, et al. Inhibition of activation-induced apoptosis of thymocytes by all-trans- and 9-cis-retinoic acid is mediated via retinoic acid receptor α. Biochem. J., 1998, 331:767-774.
[4]. Jean-Ju Chung, Sehyung Cho, Yunhee Kim Kwon, et al. Activation of retinoic acid receptor γ induces proliferation of immortalized hippocampal progenitor cells. Molecular Brain Research, 2000, 83:52-62.
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 486.6 |
Cas No. | 170355-78-9 |
Formula | C31H34O5 |
Solubility | Soluble in DMSO |
Chemical Name | 4-(7-((1s,3R,5S,7s)-adamantan-1-yl)-6-((2-methoxyethoxy)methoxy)naphthalen-2-yl)benzoic acid |
SDF | Download SDF |
Canonical SMILES | OC(C1=CC=C(C=C1)C2=CC=C3C(C=C(C(OCOCCOC)=C3)[C@]4(C[C@H](C5)C6)C[C@@H]5C[C@H]6C4)=C2)=O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |