CCT007093
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
CCT007093是一种PPM1D小分子抑制剂,IC50值为8.4 μM[1].
CCT007093是一种thienylidene环戊酮,在用重组的磷酸化-P38作为底物进行的体外试验中,CCT007093对PPM1D表现出强的抑制作用.细胞实验显示,相较HeLa细胞,CCT007093对MCF-7细胞表现出特异性.2天之后,降低了40%的细胞活力.CCT007093诱导的细胞死亡依赖于P38激酶的活性.CCT007093模仿了PPM1D RNAi激活P38激酶的作用.在MCF-7细胞中,暴露后4 h,CCT007093诱导P38的磷酸化,并且细胞活力的损失可以被特异性p38抑制剂SB203580修复[1].
参考文献：
[1] Rayter S, Elliott R, Travers J, Rowlands MG, Richardson TB, Boxall K, Jones K, Linardopoulos S, Workman P, Aherne W, Lord CJ, Ashworth A. A chemical inhibitor of PPM1D that selectively kills cells overexpressing PPM1D. Oncogene. 2008 Feb 14;27(8):1036-44.
| Storage | Store at -20°C |
| M.Wt | 272.39 |
| Cas No. | 176957-55-4 |
| Formula | C15H12OS2 |
| Solubility | insoluble in EtOH; insoluble in H2O; ≥3.4 mg/mL in DMSO |
| Chemical Name | (2Z,5E)-2,5-bis(thiophen-2-ylmethylidene)cyclopentan-1-one |
| SDF | Download SDF |
| Canonical SMILES | C1CC(=CC2=CC=CS2)C(=O)C1=CC3=CC=CS3 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| Description | CCT007093是一种有效的PPM1D (WIP1)抑制剂,IC50值为8.4 μM | |||||
| 靶点 | WIP1 | |||||
| IC50 | 8.4 μM | |||||
质量控制和MSDS
- 批次:
化学结构
