CCT 031374 hydrobromide
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
CCT 031374 hydrobromide是Wnt/β-catenin信号传导途径的选择性抑制剂[1].
Wnt配体与含有LRP5/6和Frizzled (Fz)受体的复合体结合,从而活化Wnt/β-catenin途径,然后激活转录因子/β连环蛋白依赖性转录[1].
CCT 031374 hydrobromide是Wnt/β-catenin信号通路的选择性抑制剂.在L细胞中,CCT031374抑制BIO诱导的β连环蛋白积累,IC50值为6.1 μM.在HCT116人结肠癌细胞系中,CCT031374通过诱导细胞凋亡抑制细胞增殖.在小鼠L细胞中,GSK-3抑制剂BIO显著增加总β连环蛋白含量.在核以及胞浆中,CCT031374抑制BIO诱导的β连环蛋白累积.在U2OS GFP-β连环蛋白人骨肉瘤细胞中,CCT031374诱导GFP-β连环蛋白聚集体的形成.在人类神经胚体中,CCT031374降低内源性LEF1的mRNA水平.此外,CCT031374抑制HT29\HCT116\SW480\SNU475和CCD841Co癌细胞系的细胞生长,GI值分别为11.5\13.9\13.2\9.6和44 μM[1].
参考文献：
[1]. Ewan K, Pajak B, Stubbs M, et al. A useful approach to identify novel small-molecule inhibitors of Wnt-dependent transcription. Cancer Res, 2010, 70(14): 5963-5973.
| Physical Appearance | Pale yellow solid |
| Storage | Desiccate at RT |
| M.Wt | 434.33 |
| Cas No. | 1219184-91-4 |
| Formula | C23H19N3O·HBr |
| Solubility | <21.72mg/ml in DMSO |
| Chemical Name | 1-([1,1'-biphenyl]-4-yl)-2-(2H-benzo[d]imidazo[1,2-a]imidazol-9(3H)-yl)ethanone hydrobromide |
| SDF | Download SDF |
| Canonical SMILES | O=C(C1=CC=C(C2=CC=CC=C2)C=C1)CN3C4=CC=CC=C4N5CCN=C53.Br |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
