CCG-1423
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
CCG-1423是RhoA转录信号的小分子抑制剂.MRTF-A和输入蛋白a/b1之间的相互作用可被CCG-1423抑制,但单体G-肌动蛋白结合MRTF-A不被抑制[4].
Rho家族小GTPases的多个成员在多个细胞过程和一些人类肿瘤(如结肠\食道\肺\胰腺和炎症性乳腺癌)中扮演重要角色,RhoA或RhoC的上调则与不良临床结果有关[2,3].
CCG-1423对Rho过表达和侵袭性癌细胞系有纳摩尔到低微摩尔级别的效果和选择性,可抑制DNA合成\细胞生长和/或侵袭.在高转移性的RhoC过表达的A375M2黑色素瘤细胞系中,CCG-1423可增强Caspase-3激活,但在亲本A375细胞系中的提高较小,柔红霉素的情形则正好相反[2].
参考文献：
[1]. Minami T, Kuwahara K, Nakagawa Y et al. Reciprocal expression of MRTF-A and myocardin is crucial for pathological vascular remodelling in mice. EMBO J. 2012 Nov 28;31(23):4428-40. doi: 10.1038/emboj.2012.296. Epub 2012 Oct 26.
[2]. Hayashi K, Watanabe B, Nakagawa Y et al. RPEL proteins are the molecular targets for CCG-1423, an inhibitor of Rho signaling. PLoS One. 2014 Feb 18;9(2):e89016. doi: 10.1371/journal.pone.0089016. eCollection 2014.
- 1. Shuo Chen, Fangfang Guo, et al. "Roles of the RhoA-ROCK Signaling Pathway in the Endothelial H2S Production and Vasodilation in Rat Cerebral Arteries." ACS Omega. 2022 May 20;7(22):18498-18508. PMID: 35694456
- 2. Zhao Y, Ge X, et al. "Inhibition of ROCK signaling pathway accelerates enteric neural crest cell-based therapy after transplantation in a rat hypoganglionic model." Neurogastroenterol Motil. 2020;e13895. PMID: 32515097
| Storage | Store at -20°C |
| M.Wt | 454.75 |
| Cas No. | 285986-88-1 |
| Formula | C18H13ClF6N2O3 |
| Solubility | ≥21 mg/mL in DMSO; insoluble in EtOH; insoluble in H2O |
| Chemical Name | N-((1-((4-chlorophenyl)amino)-1-oxopropan-2-yl)oxy)-3,5-bis(trifluoromethyl)benzamide |
| SDF | Download SDF |
| Canonical SMILES | FC(F)(F)C1=CC(C(F)(F)F)=CC(C(NOC(C)C(NC2=CC=C(Cl)C=C2)=O)=O)=C1 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
相关生物数据
相关生物数据
