CBB1007
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
CBB1007是组蛋白去甲基化酶LSD1的抑制剂，IC50值为5.27 μM[1]。
LSD1是FAD依赖性胺氧化酶家族的成员，将二甲基化的H3K4转换成单和非甲基化的H3K4，抑制基因表达。作为LSD1的抑制剂，CBB1007可用于研究该酶的功能。在体外实验中，CBB1007具有高效的抑制活性，IC50值为5.27 μM。CBB1007对LSD1是特异性的，对其它组蛋白去甲基化酶没有影响，包括LSD2和JARID1A。在小鼠F9胚胎畸胎瘤细胞中，CBB1007引起单甲基化和二甲基化H3K4的可重复增加，IC50值为1 μM-5 μM。CBB1007也激活CHRM4和SCN3A的表达，其是LSD1的靶基因。对LSD1的抑制导致这些细胞的生长抑制。而且，CBB1007也抑制其它多能癌细胞以及表达干细胞标记Oct4和Sox2的胚胎干细胞的增殖[1]。
参考文献：
[1] Wang J, Lu F, Ren Q, et al. Novel histone demethylase LSD1 inhibitors selectively target cancer cells with pluripotent stem cell properties. Cancer research, 2011, 71(23): 7238-7249.
| Storage | Store at -20°C |
| M.Wt | 534.61 |
| Cas No. | 1379573-92-8 |
| Formula | C27H34N8O4 |
| Solubility | Soluble in DMSO |
| Chemical Name | methyl 3-(4-(4-carbamimidoylbenzoyl)piperazine-1-carbonyl)-5-((4-carbamimidoylpiperazin-1-yl)methyl)benzoate |
| SDF | Download SDF |
| Canonical SMILES | COC(C1=CC(CN2CCN(C(N)=N)CC2)=CC(C(N3CCN(C(C4=CC=C(C(N)=N)C=C4)=O)CC3)=O)=C1)=O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
化学结构
