UK 1
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
UK 1, an unusual bis-benzoxazole metabolite isolated from Streptomyces sp. 517-02, is an inhibitor of topoisomerase II (Topo II) and hepatitis C viral replication. It is an antifungal and an antibiotic, and displays a wide spectrum of potent anticancer activities against lymphoma, leukemia, and certain solid tumor-derived cell lines with IC50 values as low as 20 nM. However, UK 1 is devoid of antimicrobial activity and is inactive against Pseudomonas aeruginosa and Staphylococcus aureus, a methicillin-resistant strain of S. aureus. Additionally, UK-1 can bind a wide variety of di- and tri-valent metal ions, particularly Mg2+ ions, and form complexes with DNA in the presence of Mg2+ ions. Topo II are ATP-dependent enzymes, which can simultaneously cut both strands of the DNA helix to manage DNA supercoils and tangles.
In vitro: UK 1 strongly blocked the growth of human epitheloid carcinoma HeLa, mouse melanoma B16, mouse leukemia P388 cells with ED50 values of 1.22 μg/ml, 1.17 μg/ml, and 0.11 μg/ml, respectively. UK 1 did not display any growth inhibitory activity against bacteria, fungi and yeasts up to 100 μg/ml [1].
In vivo: Up to now, in vivo study of UK 1 is still in the development stage.
Reference:
[1]. UEKI, M., UENO, K., MIYADOH, S., ABE, K., SHIBATA, K., TANIGUCHI, M., & OI, S. UK-1, a novel cytotoxic metabolite from Streptomyces sp. 517-02. I. Taxonomy, fermentation, isolation, physico-chemical and biological properties. The Journal of Antibiotics. 1993; 46(7): 1089-1094.
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 386.4 |
Cas No. | 151271-53-3 |
Formula | C22H14N2O5 |
Solubility | Soluble in DMSO;Soluble in dimethyl formamide |
Chemical Name | 2'-(2-hydroxyphenyl)-[2,4'-bibenzoxazole]-4-carboxylic acid, methyl ester |
SDF | Download SDF |
Canonical SMILES | OC(C=CC=C1)=C1C2=NC3=C(C=CC=C3C4=NC5=C(C=CC=C5C(OC)=O)O4)O2 |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |