Bialaphos (sodium salt)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
The antibiotic bialaphos (or SF1293) is a natural non-selective phytotoxin produced by Streptomyces hygroscopicus. Bialaphos is a tripeptide consisting of two L-alanine molecules and an L-glutamic acid analogue called phosphinothricin. Bialaphos is converted to the active agent phosphinothricin by peptidases. The phosphinothricin moiety is a potent inhibitor of glutamine synthetase [1].
Glutamine synthetase is an enzyme that plays an essential role in the metabolism of nitrogen. Glutamine synthetase in the brain participates in the metabolic regulation of glutamate, the detoxification of brain ammonia, the assimilation of ammonia, recyclization of neurotransmitters, and termination of neurotransmitter signals. Upregulation of glutamine synthetase in glutamatergic areas or other adaptations alleviates high levels of glutamate and ammonia [2].
Bialaphos has been used to select the transgenic plants that express the bar gene, usually under the control of a constitutively active viral promoter. In bialaphos resistant rice plants expressing a bar gene, treatment with the herbicide completely protected from symptomatic infection. In transgenic plants infected with R. solani two days before herbicide treatment, bialaphos induced substantial suppression of sheath blight symptoms [3]. In transgenic rice harboring the Bar-gene, treatment with bialaphos decreased the lesions caused by the fungal pathogen. Pretreatment with bialaphos 1 day before the inoculation suppressed disease symptoms to 20% of control [4].
References:
[1] Meister A. 23. Glutamine synthetase of mammals[J]. The enzymes, 1974, 10: 699-754.
[2] Murakami T, Anzai H, Imai S, et al. The bialaphos biosynthetic genes ofStreptomyces hygroscopicus: Molecular cloning and characterization of the gene cluster[J]. Molecular and General Genetics MGG, 1986, 205(1): 42-53.
[3] Uchimiya H, Iwata M, Nojiri C, et al. Bialaphos treatment of transgenic rice plants expressing a bar gene prevents infection by the sheath blight pathogen (Rhizoctonia solani)[J]. Nature Biotechnology, 1993, 11(7): 835-836.
[4] Tada T, Kanzaki H, Norita E, et al. Decreased symptoms of rice blast disease on leaves of bar-expressing transgenic rice plants following treatment with bialaphos[J]. MPMI-Molecular Plant Microbe Interactions, 1996, 9(8): 762.
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 345.26 |
Cas No. | 71048-99-2 |
Formula | C11H21N3NaO6P |
Solubility | Soluble in DMSO |
Chemical Name | sodium (2S)-2-((2S)-2-((2S)-2-amino-4-(hydroxy(methyl)phosphoryl)butanamido)propanamido)propanoate |
SDF | Download SDF |
Canonical SMILES | [O-]C([C@@H](NC([C@@H](NC([C@H](CCP(C)(O)=O)N)=O)C)=O)C)=O.[Na+] |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |