β-Glycerophosphate (sodium salt hydrate)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
β-Glycerophosphate (sodium salt hydrate) is a potent protein phosphatase inhibitor [1].
Protein phosphatase is an enzyme that removes a phosphate group from the phosphorylated amino acid residue of its target protein. Protein phosphorylation is one of the most common protein posttranslational modifications.
β-Glycerophosphate is a potent protein phosphatase inhibitor that acts as a phosphate group donor in bone cell mineralization studies [1][3]. In MC3T3-E1, ROS 17/2.8, and chick osteoblast-like cells, β-Glycerophosphate did not affect the rate of anaerobic glycolysis, but increased the medium Pi levels, suggesting that β-Glycerophosphate was hydrolyzed by bone cells. The local increase in medium Pi concentration promoted rapid mineral deposition [1]. In human bone marrow stromal cells (HBMSC) and human osteoblasts (HOB), BMP-2 resulted in mineralization of their matrix in the presence of beta-glycerophosphate and ascorbic acid [3]. β-Glycerophosphate (10 mM) also accelerated calcification of cultured vascular smooth muscle cells through an alkaline phosphatase-related mechanism [4].
References:
[1]. Chung CH, Golub EE, Forbes E, et al. Mechanism of action of beta-glycerophosphate on bone cell mineralization. Calcif Tissue Int. 1992 Oct;51(4):305-11.
[2]. Fujimoto H, Mabuchi I. Isolation of cleavage furrows from eggs of regular sea urchins and identification of furrow-specific proteins. J Biochem. 1997 Sep;122(3):518-24.
[3]. Lecanda F, Avioli LV, Cheng SL. Regulation of bone matrix protein expression and induction of differentiation of human osteoblasts and human bone marrow stromal cells by bone morphogenetic protein-2. J Cell Biochem. 1997 Dec 1;67(3):386-96.
[4]. Shioi A, Nishizawa Y, Jono S, et al. Beta-glycerophosphate accelerates calcification in cultured bovine vascular smooth muscle cells. Arterioscler Thromb Vasc Biol. 1995 Nov;15(11):2003-9.
- 1. Huazhi Zhang, Zhihui Cui, et al. "RNF186/EPHB2 Axis Is Essential in Regulating TNF Signaling for Colorectal Tumorigenesis in Colorectal Epithelial Cells." J Immunol. 2022 Nov 1;209(9):1796-1805. PMID: 36130827
- 2. Yu Wang, Shanshan Jin, et al. "Functional regeneration and repair of tendons using biomimetic scaffolds loaded with recombinant periostin." Nat Commun. 2021 Feb 26;12(1):1293. PMID: 33637721
- 3. Huazhi Zhang, Zhihui Cui, et al. "RNF186 regulates EFNB1 (ephrin B1)-EPHB2-induced autophagy in the colonic epithelial cells for the maintenance of intestinal homeostasis." Autophagy. 2021 Oct;17(10):3030-3047. PMID: 33280498
Storage | Store at -20°C |
M.Wt | 306.1 |
Cas No. | 13408-09-8 |
Formula | C3H7O6P·2Na [5H2O] |
Solubility | insoluble in EtOH; ≥14.3 mg/mL in DMSO; ≥30.7 mg/mL in H2O |
Chemical Name | 2-(dihydrogen phosphate)-1,2,3-propanetriol, disodium salt, pentahydrate |
SDF | Download SDF |
Canonical SMILES | [O-]P(OC(CO)CO)([O-])=O.[Na+].[Na+].O.O.O.O.O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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