Ampkinone
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Ampkinone is an AMPK activator.
Adenosine 5'-monophosphate (AMP) activated protein kinase (AMPK) has considered as an promising target molecule for the treatment of metabolic disorders, such as obesity and type 2 diabetes.
In vitro: Ampkinone was identified as an indirect AMPK activator, which was derived from the small molecule library constructed by diversity-oriented synthesis. Ampkinone was able to stimulate the phosphorylation of AMPK through the indirect activation of AMPK in various cell lines. Moreover, ampkinone-mediated activation of AMPK needed the activity of LKB1 and led to increased glucose uptake in muscle cells [1].
In vivo: Animal study found that ampkinone-treated DIO mice had significantly reduced total body weight and overall fat mass. Histological examination and measurement of lipid parameters showed that ampkinone could effectively improve metabolic abnormalities. These results demonstrated that ampkinone had a potential as a new class of therapeutic agent for antidiabetic and antiobesity treatment through the indirect stimulation of AMPK [1].
Clinical trial: So far, no clinical study has been conducted.
Reference:
[1] Oh, S. ,Kim, S.J.,Hwang, J.H., et al. Antidiabetic and antiobesity effects of ampkinone (6f), a novel small molecule activator of AMP-activated protein kinase. Journal of Medicinal Chemistry 53, 7405-7413 (2010).
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 505.5 |
Cas No. | 1233082-79-5 |
Formula | C31H23NO6 |
Solubility | ≤2mg/ml in DMSO;10mg/ml in dimethyl formamide |
Chemical Name | 2-(4-benzoylphenyl)-6-hydroxy-7-methoxy-4,4-dimethyl-[1]benzopyrano[3,4-e]isoindole-1,3(2H,4H)-dione |
SDF | Download SDF |
Canonical SMILES | O=C1N(C2=CC=C(C(C3=CC=CC=C3)=O)C=C2)C(C4=C1C=CC5=C4C(C)(C)OC6=C(O)C(OC)=CC=C65)=O |
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