2-fluoro Palmitic Acid
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
2-fluoropalmitic acid inhibits sphingosine biosynthesis and long-chain acyl-CoA synthetase [1]. The length of the carbon chain of the fatty acid species defines the substrate specificity for the different acyl-CoA synthetases (ACS).
Mammalian long-chain acyl-CoA synthetases (ACSL) activate fatty acids with chain lengths of 12 to 20 carbon atoms. The long-chain acyl-CoA synthetase mRNA is expressed virtually in heart, liver, and epididymal adipose tissues and, to a much lesser extent, in brain, small intestine, and lung [2].
Palmitic acid was a selective cytotoxic substance extracted from the marine algal. At concentrations ranging from 12.5 to 50 μg/ml, palmitic acid showed selective cytotoxicity to human leukemic cells, but no cytotoxicity to normal HDF cells. Palmitic acid (50 μg/ml) induced apoptosis in the human leukemic cell line MOLT-4. Palmitic acid also showed in vivo antitumor activity in mice [3]. 2-fluoropalmitic acid showed an inhibitory effect on sphingosine biosynthesis and long-chain acyl-CoA synthetase with an IC50 value of 0.2 mM [1].
References:
[1] Soltysiak R M, Matsuura F, Bloomer D, et al. d, l-α-fluoropalmitic acid inhibits sphingosine base formation and accumulates in membrane lipids of cultured mammalian cells[J]. Biochimica et Biophysica Acta (BBA)-Lipids and Lipid Metabolism, 1984, 792(2): 214-226.
[2] Suzuki H, Kawarabayasi Y, Kondo J, et al. Structure and regulation of rat long-chain acyl-CoA synthetase[J]. Journal of Biological Chemistry, 1990, 265(15): 8681-8685.
[3] Harada H, Yamashita U, Kurihara H, et al. Antitumor activity of palmitic acid found as a selective cytotoxic substance in a marine red alga[J]. Anticancer research, 2001, 22(5): 2587-2590.
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 274.4 |
Cas No. | 16518-94-8 |
Formula | C16H31FO2 |
Solubility | Soluble in DMSO |
Chemical Name | 2-fluorohexadecanoic acid |
SDF | Download SDF |
Canonical SMILES | CCCCCCCCCCCCCCC(F)C(=O)O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |