CUDA
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
IC50: 11.1 and 112 nM for the mouse and human soluble epoxide hydrolase, respectively
CUDA is a soluble epoxide hydrolase (sEH) inhibitor.
Epoxyeicosatrienoic acid metabolites of arachidonic acid, such as 11(12)-EET and 14(15)-EET, have been identified as endothelium derived hyperpolarizing factors with vasodilator activity. Soluble epoxide hydrolase (sEH) can catalyze the conversion of EETs to the corresponding dihydroxy eicosatrienoic acids thereby diminishing their activity.
In vitro: In a previous study, in order to test if the CUDA’s mechanism of action is retained upon structural modification, the dissociation constants of CUDA was evaluated for mouse sEH. Results showed that for CUDA, competitive a tight-binding inhibition kinetic was obtained with r2 > 0.99. Moreover, the KI value of 3.8 nM was obtained for CUDA. In addition, it was found that CUDA was an inhibitor of sEH exhibiting IC50 values of 11.1 nM and 112 nM for the mouse and human enzymes, respectively [1].
In vivo: Currently, there is no animal in vivo data reported.
Clinical trial: So far, no clinical study has been conducted.
Reference:
[1] C. Morisseau, M. H. Goodrow, J. W. Newman, et al. Structural refinement of inhibitors of urea-based soluble epoxide hydrolases. Biochemical Pharmacology 63, 1599-1608 (2002).
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 340.5 |
Cas No. | 479413-68-8 |
Formula | C19H36N2O3 |
Solubility | ≤1mg/ml in ethanol;5mg/ml in DMSO;10mg/ml in dimethyl formamide |
Chemical Name | 12-[[(cyclohexylamino)carbonyl]amino]-dodecanoic acid |
SDF | Download SDF |
Canonical SMILES | O=C(NCCCCCCCCCCCC(O)=O)NC1CCCCC1 |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |