L-Homopropargyl Glycine
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
L-homopropargylglycine (Hpg), an alkyne-tagged analog of methionine, has been used to selectively tag newly synthesized mammalian proteins in a method similar to conventional pulse-chase labeling, and labeled proteins were visualized via fluorescence. Protein translation has been implicated in different forms of synaptic plasticity, but direct in situ visualization of new proteins is limited to one or two proteins at a time. Homopropargylglycine is a noncanonical amino acid which can incorporate into proteins followed by chemoselective fluorescence tagging by means of 'click chemistry'. Pulse-chase application of homopropargylglycine allowed visualization of proteins synthesized in two sequential time periods. This technique can be used to detect changes in protein synthesis and to evaluate the fate of proteins synthesized in different cellular compartments [1].
Reference:
[1] Dieterich D C, Hodas J J L, Gouzer G, et al. In situ visualization and dynamics of newly synthesized proteins in rat hippocampal neurons[J]. Nature neuroscience, 2010, 13(7): 897-905.
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 127.1 |
Cas No. | 98891-36-2 |
Formula | C6H9NO2 |
Synonyms | Click Tag™ L-Homopropargyl Glycine,HPG |
Solubility | ≤10mg/ml in PBS(pH7.2) |
Chemical Name | (2S)-2-amino-5-hexynoic acid |
SDF | Download SDF |
Canonical SMILES | C#CCC[C@H](N)C(O)=O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |