Tetradecyl Phosphonate
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Tetradecyl phosphonate is a pan-antagonist of lysophosphatidic acid 1 (LPA1), LPA2, and LPA3 receptors. The IC50 value for inhibition of LPA-induced calcium mobilization was 10 μM, 5.5 μM, and 3.1 μM, respectively. At a concentration of 10 μM, tetradecyl phosphonate activates a peroxisome proliferator-activated receptor γ reporter construct 4-fold when compared with the controls and partially inhibits autotaxin with an IC50 value of approximately 3 μM [1].
Lysophosphatidic acid (LPA), also known as autotaxin (ATX), is a lipid signalling molecule formed by the hydrolysis of lysophosphatidyl choline by lysophospholipase D [2]. LPA signals through four different G protein-coupled receptors, named as LPA1/EDG-2, LPA2/EDG-4, LPA3/EDG-7, and LPA4/GPR23 [3]. It has been reported that LPA was involved in activating peroxisome proliferator-activated receptor γ (PPARγ) [4].
References:
[1]. Durgam G G, Virag T, Walker M D, et al. Synthesis, structure activity relationships, and biological evaluation of fatty alcohol phosphates as lysophosphatidic acid receptor ligands, activators of PPARγ, and inhibitors of autotaxin[J]. Journal of medicinal chemistry, 2005, 48(15): 4919-4930.
[2]. Tokumura A, Majima E, Kariya Y, et al. Identification of human plasma lysophospholipase D, a lysophosphatidic acid-producing enzyme, as autotaxin, a multifunctional phosphodiesterase[J]. Journal of Biological Chemistry, 2002, 277(42): 39436-39442.
[3]. Noguchi K, Ishii S, Shimizu T. Identification of p2y9/GPR23 as a novel G protein-coupled receptor for lysophosphatidic acid, structurally distant from the Edg family[J]. Journal of Biological Chemistry, 2003, 278(28): 25600-25606.
[4]. McIntyre T M, Pontsler A V, Silva A R, et al. Identification of an intracellular receptor for lysophosphatidic acid (LPA): LPA is a transcellular PPARγ agonist[J]. Proceedings of the National Academy of Sciences, 2003, 100(1): 131-136.
Storage | Store at -20°C |
M.Wt | 278.4 |
Cas No. | 4671-75-4 |
Formula | C14H31O3P |
Solubility | ≥27.8 mg/mL in ETOH; insoluble in H2O; ≥2.27 mg/mL in DMSO with gentle warming and ultrasonic |
Chemical Name | tetradecylphosphonic acid |
SDF | Download SDF |
Canonical SMILES | CCCCCCCCCCCCCCP(=O)(O)O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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