RHPS4
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
在5天生长抑制测定中,作用于M14细胞,IC50约为3 μM。
RHPS4是一种端粒酶抑制剂。
端粒调节染色体末端的保护、降解和重组。近期一项模型提出端粒可以在染色体末端形成帽。因此,端粒帽完整性应该是完整的,以允许细胞分裂进行。
体外:研究发现,RHPS4处理UXF1138L细胞导致端粒酶催化亚基(hTERT)从细胞核置换、端粒启动的DNA损伤信号传导以及染色体融合的诱导。进一步报道,RHPS4对作为集落生长的癌细胞比作为单层生长的细胞更有效。此外,人脐带血和HEK293T胚胎肾细胞集落形成单位对RHPS4更耐受[1]。
体内:动物研究发现,与对照相比,RHPS4处理UXF1138L异种移植物可降低克隆形成,使核hTERT表达丧失,并诱导有丝分裂异常。虽然RHPS4单独用药表现出有限的体内功效,与有丝分裂纺锤体毒物Taxol的联合用药治疗导致肿瘤缓解和端粒功能障碍的进一步增强[1]。
临床试验:到目前为止,RHPS4仍处于临床前研究阶段。
参考文献:
[1] Phatak P,Cookson JC,Dai F,Smith V,Gartenhaus RB,Stevens MF,Burger AM. Telomere uncapping by the G-quadruplex ligand RHPS4 inhibits clonogenic tumour cell growth in vitro and in vivo consistent with a cancer stem cell targeting mechanism. Br J Cancer.2007 Apr 23;96(8):1223-33.
- 1. Chen H, Sun H, et al. "Monitoring autophagy in live cells with a fluorescent light-up probe for G-quadruplex structures." Chem Commun (Camb). 2019 Apr 25;55(35):5060-5063. PMID:30907894
- 2. SugeZhang, HongxiaSun, et al. "Evaluation of the selectivity of G-quadruplex ligands in living cells with a small molecule fluorescent probe." Analytica Chimica Acta: X. Available online 23 April 2019, 100017.
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 458.48 |
Cas No. | 390362-78-4 |
Formula | C23H20F2N2O4S |
Solubility | insoluble in EtOH; ≥10.54 mg/mL in DMSO; ≥2.51 mg/mL in H2O with ultrasonic |
Chemical Name | 3,11-difluoro-6,8,13-trimethyl-8H-quinolino[4,3,2-kl]acridin-13-ium methyl sulfate |
SDF | Download SDF |
Canonical SMILES | FC1=CC=C(N(C)C2=C3C4=[N+](C)C5=C(C=C(F)C=C5)C3=CC(C)=C2)C4=C1.COS(=O)([O-])=O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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