Protein A/G
Catalog No.
P1090
蛋白A/G,用于纯化IgG抗体,在大肠杆菌中表达。
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Protein A is a 42 kDa surface protein originally found in the cell wall of the bacterium Staphylococcus aureus. It can bind immunoglobulins. Protein G is an immunoglobulin-binding protein expressed in group C and G Streptococcal bacteria. Protein A/G is a genetically engineered protein that combines the IgG binding profiles of both Protein A and Protein G. It is a gene fusion product. Recombinant fusion protein A/G contains 6× His-tag on the N-terminus, five Ig-binding regions of protein A fusion with three Ig-binding region of protein G. Cell wall binding region, albumin binding region and other non-specific binding regions have been eliminated from the fusion protein A/G to ensure the maximum specific IgG binding. 6× His-tag on N-terminus can be used for affinity purification or for protein A/G detection using anti-His-tag antibody.
Protein A/G binds to all IgG subclasses from various mammalian species, including all IgGs that bind to both Protein A and Protein G, making it the ideal choice for purification of all kinds of polyclonal or monoclonal IgG antibodies.
Quality Control & DataSheet
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Purity: ≥98%, by SDS-PAGE and HPLC.
Endotoxin Level:<0.1 ng/μg
Gene ID: | N/A | Accession #: | N/A |
Alternate Names: | Proteina/g, protein ag, protein a/g, proteinag, protein-ag, protein-a/g, proteinag, Protein A/G, rProtein A/G, r-protein A/G, recombinant protein A/G, IgG purification, antibody purification, Protein A, Protein G, Protein A&G. | ||
Source: | Escherichia Coli. | M.Wt: | 59.7 kDa |
Appearance: | Lyophilized protein | Storage: | Store at -20°C |
Formulation: | Lyophilized powder. | ||
Solubility: | Reconstitution in water to a concentration of 5 mg/ml gives a clear solution.. | ||
Shipping Condition: | Gel pack. | Handling: | Centrifuge the vial prior to opening. |
Usage: | For Research Use Only! Not to be used in humans. |