Annexin V-Biotin Apoptosis Kit
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Annexin V is a cellular protein, which plays important roles in the inhibition of the activity of phospholipase A1 and blood coagulation by competing for phosphatidylserine (PS) binding sites with prothrombin. Annexin V has a high affinity to PS and is used as a probe to detect cells that have expressed PS on the cell surface. After initiating apoptosis, cells translocate phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface soon, which can be easily detected by Annexin V.
Annexin V-Biotin Apoptosis Detection Kit uses Biotin-conjugated Annexin V that can easily detect PS on the cell surface after initiating apoptosis. Annexin V-Biotin can be easily detected in combination with conventional dye-staining using any avidin- or streptavidine-dye reagents, such as (strept)avidine-peroxidase, -fluorescein, -alkaline phosphatase (AP), and -β-gal, etc. The result can be analyzed by fluorescence microscopy or by flow cytometry.
Features | Fast and convenient Simple one step staining procedure in 30 minutes. Annexin V-Biotin can be detected in conjunction with conventional dye-staining using any streptavidine- or avidin-dye reagents, such as (strept) avidine-fluorescein, -peroxidase, -alkaline phosphatase (AP), and -β-gal, et |
Shipping | Gel pack |
Storage Conditions | Store at +4°C. |
Related Biological Data
Related Biological Data
Related Biological Data
Species reactivity | Mammalian |
Applications | Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis. |
Kit components | Annexin V-Biotin 1X Binding Buffer Propidium Iodide (PI) |