Caspase-3 Colorimetric Assay Kit
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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
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Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
含半胱氨酸的天冬氨酸蛋白水解酶(Caspase) 是一个半胱氨酸蛋白酶家族，在细胞凋亡、坏死和炎症中起重要作用。 Caspase 的连续激活在细胞凋亡中起重要作用。 Caspase-3 是一种 caspase 蛋白，可切割和激活 caspase-6 和 -7，并被 caspase-8、-9 和 -10 加工和激活。 Caspase-3 是一种重要的caspase 蛋白，参与阿尔茨海默病相关的淀粉样 β4A 前体蛋白的裂解。 Caspase-3 识别四肽序列 D-x-x-D 并水解天冬氨酸残基后的肽键。
Caspase-3 Colorimetric Assay Kit为检测 DEVD 依赖性 Caspase 活性提供了一种方便且简单的方法。当 Caspase-3 或相关的半胱天冬酶从标记的底物 DEVD-p-硝基苯胺 (DEVD-pNA) 上裂解 DEVD 时，可以使用微量滴定板阅读器或分光光度计在 405 或 400 nm 处量化游离 pNA 的光发射。将凋亡样品中游离 pNA 的吸光度与未诱导的对照进行比较，确定 Caspase-3 活性的增加倍数。
| Features | Fast and convenient Simple one-step procedure; takes 1-2 hours. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in CPP32 activity. |
| Shipping | Gel pack |
| Storage Conditions | Store at -20°C. |
Related Biological Data
Related Biological Data
Related Biological Data
Related Biological Data
| Species reactivity | Mammalian |
| Applications | Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis. |
| Kit components | Cell Lysis Buffer 2X Reaction Buffer DEVD-pNA (4 mM) DTT (1 M) |
| Components | K2008-25 25 assays |
K2008-100 100 assays |
K2008-200 200 assays |
K2008-400 400 assays |
Part Number |
| Cell Lysis Buffer | 25 ml | 100 ml | 100 ml | 100 ml | K2008-C-1 |
| 2 X Reaction Buffer | 2 ml | 4 x 2 ml | 16 ml | 32 ml | K2008-C-2 |
| DEVD-pNA (4 mM) | 125 μl | 500 μl | 2 x 0.5 ml | 2 x 1 ml | K2008-C-3 |
| DTT (1 M) | 100 μl | 400 μl | 400 μl | 400 μl | K2008-C-4 |
-20°C保存 | |||||