Oncostatin M, human recombinant protein
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Oncostatin (OSM) is a pleiotropic cytokine produced by activated T cells, monocytes, and Kaposi’ s sarcoma cells. OSM stimulates the proliferation of fibroblasts, smooth muscle cells and Kaposi’s sarcoma cells, inhibits the growth of some normal and tumor cell lines, promotes cytokine release (IL-6, GM-CSF and G-CSF) from endothelial cells, and enhances the expression of low-density lipoprotein receptors in hepatoma cells.
Human OSM cDNA encodes a 252 amino acid pre-pro-OSM polypeptide with a 25 residue signal peptide and a hydrophilic C-terminal domain that are proteolytically processed to generate the 196 residue mature OSM protein. Human OSM is active on murine cells. Recombinant human Oncostatin M is a 26 kDa protein containing 227 amino acid residues.
| Determined by the dose-dependent stimulation of the proliferation of human TF-1 cells. | ||||||
| ED50 | <2 ng/ml. | |||||
| Specific activity | > 5*105 units/mg. | |||||
Quality Control & DataSheet
- View current batch:
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Purity: ≥98%, by SDS-PAGE and HPLC.
Endotoxin Level:< 0.1 ng/μg/span>
相关生物数据
相关生物数据
| Gene ID: | 5008 | Accession #: | P13725 |
| Alternate Names: | Human Oncostatin M, h-Oncostatin M, rh-Oncostatin M, recombinant human Oncostatin M, recombinant Oncostatin M. | ||
| Source: | Escherichia Coli. | M.Wt: | 26.0 kDa |
| Appearance: | Lyophilized protein | Storage: | Store at -20°C |
| Formulation: | Sterile filtered and lyophilized with no additives. | ||
| Solubility: | Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use. | ||
| Shipping Condition: | Gel pack. | Handling: | Centrifuge the vial prior to opening. |
| Usage: | For Research Use Only! Not to be used in humans. | ||