IL-21, human recombinant protein
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Human IL-21 is encoded by IL-21 gene located on Chr. 4. It is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation and can regulating immune system cells, for instance cytotoxin T cells and natural killer cells. Additionally, it can induce target cells division or proliferation. IL-21 elicits its effect through binding to IL-21R, which also contains the gamma chain found in other cytokine receptors such as IL-2, IL-4, IL-7, IL-9 and IL-15. IL-21/IL-21R interaction triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3. IL-21 shows having much relation with clinical illnesses, including cancer immunotherapy, viral infections and allergies.
Reference:
1. Pistoia VandCocco C. 2009. J Leukoc Biol, 85: 739-43
2. Ertelt JM, Johanns TM, Rowe JH, et al. 2010. Immunology, 131: 183-91
3. Denman CJ, Senyukov VV, Somanchi SS, et al. 2012. PLoS One, 7: e30264
4. Spolski R, Wang L, Wan CK, et al. 2012. J Immunol, 188: 1924-32.
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Gene ID |
59067 |
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Accession # |
Q9HBE4 |
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Alternate Names |
Za11 |
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Source |
Escherichia coli. |
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M.Wt |
Approximately 15.4 kDa, a single non-glycosylated polypeptide chain containing 133 amino acids. |
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AA Sequence |
QGQDRHMIRM RQLIDIVDQL KNYVNDLVPE FLPAPEDVET NCEWSAFSCF QKAQLKSANT GNNERIINVS IKKLKRKPPS TNAGRRQKHR LTCPSCDSYE KKPPKEFLER FKSLLQKMIH QHLSSRTHGS EDS |
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Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
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Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
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Formulation |
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4. |
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Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
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Biological Activity |
Fully biologically active when compared to standard. Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED50 for this effect is ≤ 2 ng/mL. The specific activity of recombinant human IL-21 > 5.0 × 105 U/mg. |
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Shipping Condition |
Gel pack. |
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Handling |
Centrifuge the vial prior to opening. |
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Usage |
For Research Use Only! Not to be used in humans. |
Quality Control & DataSheet
- View current batch:
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Purity: > 97 % by SDS-PAGE and HPLC analyses.
- Datasheet
Endotoxin Level: Less than 1 EU/µg of rHuIL-21 as determined by LAL method.
相关生物数据
相关生物数据
