BDNF, human recombinant
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
脑源性神经营养因子 （BDNF） 是神经营养因子（也称为神经营养因子）的 NGF 家族的成员，是中枢和周围神经系统中特定神经元亚群分化和存活所必需的。神经营养因子家族由至少四种蛋白质组成，包括 NGF、BDNF、NT-3 和 NT-4/5。这些分泌的细胞因子被合成为前肽，经过蛋白水解处理以产生成熟蛋白质。所有神经营养因子都有六个保守的半胱氨酸残基，它们参与三个二硫键的形成，并且在氨基酸水平上都具有大约 55% 的序列同一性。与NGF类似，生物活性BDNF被预测为非共价连接的同源二聚体。
| Gene ID | |
| Accession # | |
| Alternate Names | |
| Source | Spodoptera frugiperda, Sf 21 (baculovirus) |
| M.Wt | Apparent molecular mass of 13-14 kDa in SDS-PAGE under reducing conditions, a single glycosylated polypeptide protein consisting of 119 amino acids. |
| AA Sequence | His129-Arg247; Accession # P23560 |
| Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Formulation | Lyophilized from 0.2 µm filtered concentrated solution in 100 mM Sodium Citrate and 300 mM NaCl, pH 3.0. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile PBS to a concentration of 0.1 mg/mL. Further dilutions should be made in appropriately buffered solutions. |
| Biological Activity | Testing in progress.Measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB. The ED50 for this effect is 0.2-2 ng/mL. The specific activity of rHu/Mu/Rt/Ca/EqBDNF is approximately 1.3 × 103 units/μg, which is calibrated against recombinant human BDNF WHO Standard. Measured by its binding ability in a functional ELISA. When Recombinant Human TrkB Fc Chimera is coated at 1 μg/mL, rHu/Mu/Rt/Ca/EqBDNF binds with an apparent Kd <1 nM. |
| Shipping Condition | Gel pack. |
| Handling | Centrifuge the vial prior to opening. |
| Usage | For Research Use Only! Not to be used in humans. |
质量控制和DataSheet
- 批次:
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纯度 > 97 % by SDS-PAGE analyses.
- Datasheet
Endotoxin: Less than 0.1 EU/µg of rHu/Mu/Rt/Ca/EqBDNF as determined by LAL method.