Phosphocreatine disodium salt
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Phosphocreatine (also known as creatine phosphate) disodium salt is a phosphorylated creatine molecule that may serve as a quickly accessible reserve of high-energy phosphates in skeletal muscle, myocardium as well as the brain. Phosphocreatine can offer a phosphate group to ADP for ATP synthesis, or conversely, excess ATP can be used to convert creatine to creatine phosphate via creatine kinase. Thus, phosphocreatine disodium salt could be used as a substrate for the determination of creatine kinase, and is used to regenerate ATP during skeletal muscle contraction.
References:
1. Wallimann T, Wyss M, Brdiczka D, et al. Intracellular compartmentation, structure and function of creatine kinase isoenzymes in tissues with high and fluctuating energy demands: the 'phosphocreatine circuit' for cellular energy homeostasis. Biochemistry Journal, 1992, 281: 21-40.
2. Feldman EB. Creatine: a dietary supplement and ergogenic aid. Nutrition Reviews, 1999, 57(2): 45-50.
3. Qaed E, Wang J, Almoiliqy M, et al. Phosphocreatine improves cardiac dysfunction by normalizing mitochondrial respiratory function through JAK2/STAT3 signaling pathway in vivo and in vitro. Oxidative Medicine and Cellular Longevity, 2019, 2019: 6521218.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 255.08 |
| Cas No. | 922-32-7 |
| Formula | C4H8N3Na2O5P |
| Solubility | insoluble in ETOH; insoluble in DMSO; ≥54.6 mg/mL in H2O |
| Chemical Name | sodium (E)-(amino((carboxymethyl)(methyl)amino)methylene)phosphoramidate |
| SDF | Download SDF |
| Canonical SMILES | OC(CN(C)/C(N)=N/P([O-])([O-])=O)=O.[Na+].[Na+] |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| Cell experiment:[3] | |
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Cell lines |
H9C2 cells |
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Reaction Conditions |
5 ~ 20 mM phosphocreatine for 2 h incubation |
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Applications |
Phosphocreatine was found to provide a significant protective effect in H9C2 cells injured by exposure to methylglyoxal. Moreover, phosphocreatine at different concentrations (5 ~ 40 mM) has no lethality on normal H9C2 cells. |
| Animal experiment:[3] | |
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Animal models |
Wistar male rats with type 1 diabetes mellitus |
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Dosage form |
20 or 50 mM Administered intravenously once daily for 12 weeks |
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Applications |
In diabetic rats, phosphocreatine significantly reduced heart sizes, reduced blood glucose levels, and improved the striation of myocardial fibers arranged regularly in treated groups. Phosphocreatine offered a defensive impact against diabetic cardiomyopathy. |
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Note |
The technical data provided above is for reference only. |
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References: 1. Wallimann T, Wyss M, Brdiczka D, et al. Intracellular compartmentation, structure and function of creatine kinase isoenzymes in tissues with high and fluctuating energy demands: the 'phosphocreatine circuit' for cellular energy homeostasis. Biochemistry Journal, 1992, 281: 21-40. 2. Feldman EB. Creatine: a dietary supplement and ergogenic aid. Nutrition Reviews, 1999, 57(2): 45-50. 3. Qaed E, Wang J, Almoiliqy M, et al. Phosphocreatine improves cardiac dysfunction by normalizing mitochondrial respiratory function through JAK2/STAT3 signaling pathway in vivo and in vitro. Oxidative Medicine and Cellular Longevity, 2019, 2019: 6521218. |
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化学结构
