Nigericin sodium salt
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Nigericin sodium salt是一种离子载体，交换K+和H+穿过生物膜。
离子载体是一种脂溶性分子，运输离子穿过细胞膜。
在含Na+的培养基中，nigericin对ADP诱导的血小板聚集几乎没有影响，轻微抑制凝血酶诱导的血小板聚集。此外，nigericin也降低细胞质pH值。Nigericin对血小板聚集的影响主要是由于其对细胞质pH的影响[1]。K+离子载体nigericin作为Pb2+的离子载体非常有效。生理浓度的Ca2+或Mg2+不抑制nigericin催化的Pb2+运输，而K+和Na+浓度（0-100 mM）小幅影响Pb2+运输。Nigericin可能有助于治疗铅中毒[2]。在低浓度（5 μM）和高浓度（1.5 mM）ATP下，nigericin均抑制ATP驱动的转氢反应，在低ATP浓度下效果更显著。在2.5 mM ATP浓度下，nigericin诱导三倍的羰醇反应[3]。
参考文献：
[1]. Kitagawa S, Awai M, Kametani F. Relationship of the effects of nigericin on the aggregation and cytoplasmic pH of bovine platelets in the presence of different cations. Biochim Biophys Acta, 1987, 930(1): 48-54.
[2]. Hamidinia SA, Tan B, Erdahl WL, et al. The ionophore nigericin transports Pb2+ with high activity and selectivity: a comparison to monensin and ionomycin. Biochemistry, 2004, 43(50): 15956-15965.
[3]. Eytan GD, Carlenor E, Rydström J. Energy-linked transhydrogenase. Effects of valinomycin and nigericin on the ATP-driven transhydrogenase reaction catalyzed by reconstituted transhydrogenase-ATPase vesicles. J Biol Chem, 1990, 265(22): 12949-12954.
- 1. Yuan-Li Ai, Wei-Jia Wang, et al. "Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P." Cell Res. 2023 Jul 17. PMID: 37460805
- 2. Schwartz, Hannah, et al. "In vitro Methods to Better Evaluate Drug Responses in Cancer." UMass Chan Medical School. September 8, 2022.
- 3. Wang J, Luo J, et al. "Increased intracellular Cl(-) concentration by activating FAK promotes airway epithelial BEAS-2B cells proliferation and wound healing." Arch Biochem Biophys. 2019 Dec 12:108225. PMID: 31838119
- 4. Antonopoulos, Christina, et al. "Caspase-8 as an Effector and Regulator of NLRP3 Inflammasome Signaling." Journal of Biological Chemistry (2015): jbc-M115. PMID: 26100631
| Storage | Store at -20°C |
| M.Wt | 746.94 |
| Cas No. | 28643-80-3 |
| Formula | C40H67NaO11 |
| Solubility | insoluble in H2O; insoluble in DMSO; ≥74.7 mg/mL in EtOH |
| Chemical Name | sodium (S)-2-((2R,3S,6R)-6-(((2S,4S,5R,7R,9S,10R)-2-((2R,2'R,3'S,5R,5'R)-5'-((2S,3S,5R,6R)-6-hydroxy-6-(hydroxymethyl)-3,5-dimethyltetrahydro-2H-pyran-2-yl)-2,3'-dimethyloctahydro-[2,2'-bifuran]-5-yl)-9-methoxy-2,4,10-trimethyl-1,6-dioxaspiro[4.5]decan-7- |
| SDF | Download SDF |
| Canonical SMILES | O[C@@]1(CO)O[C@@H]([C@@H](C)C[C@H]1C)[C@@H]2O[C@H]([C@@H](C)C2)[C@]3(C)O[C@H](CC3)[C@@]4(C)O[C@]([C@@H](C)C4)([C@@H]5C)O[C@H](C[C@@H]6O[C@@H]([C@@H](C([O-])=O)C)[C@@H](C)CC6)C[C@@H]5OC.[Na+] |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| 细胞实验 [1]: | |
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细胞系 |
血小板 |
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制备方法 |
在DMSO中的溶解度<3.7 mg/mL。若配制更高浓度的溶液，一般步骤如下：请将试管置于37°C加热10分钟和/或将其置于超声波浴中震荡一段时间。原液于-20°C可放置数月。 |
|
反应条件 |
2 μM；2分钟 |
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实验结果 |
在含K+（与离子载体结合，具有最高渗透性）的培养基中，Nigericin Sodium Salt增强血小板聚集。然而，在含胆碱（与离子载体结合，具有较低的渗透性）的培养基中，Nigericin Sodium Salt抑制血小板聚集。此外，相对于血小板聚集率，Nigericin Sodium Salt对血小板聚集最大值的影响较大。 |
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References: [1]. Kitagawa S, Awai M, Kametani F. Relationship of the effects of nigericin on the aggregation and cytoplasmic pH of bovine platelets in the presence of different cations. Biochim Biophys Acta, 1987, 930(1): 48-54. |
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质量控制和MSDS
- 批次:
化学结构
相关生物数据
相关生物数据
相关生物数据
