Apilimod
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 418.49 |
Cas No. | 541550-19-0 |
Formula | C23H26N6O2 |
Solubility | insoluble in H2O; ≥17.9 mg/mL in DMSO; ≥6.86 mg/mL in EtOH with ultrasonic |
Chemical Name | (E)-4-(6-(2-(3-methylbenzylidene)hydrazinyl)-2-(2-(pyridin-2-yl)ethoxy)pyrimidin-4-yl)morpholine |
SDF | Download SDF |
Canonical SMILES | CC1=CC(/C([H])=N/NC2=CC(N3CCOCC3)=NC(OCCC4=CC=CC=N4)=N2)=CC=C1 |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
细胞实验[1]: | |
细胞系 |
人和猴PBMCs |
溶解方法 |
在DMSO的溶解度>17.9mg/mL。为了获得更高的浓度,可以将离心管在37℃加热10分钟和/或在超声波浴中震荡一段时间。原液可以在-20℃以下储存几个月。 |
反应条件 |
8-1000 nM |
应用 |
Apilimod抑制人PBMC中IFN-/SAC或SAC诱导的IFN-r产生。 |
动物实验[2]: | |
动物模型 |
接受人间接触感受器类视黄醇结合蛋白肽(IRBP1–20)免疫的C57BL/6J小鼠 |
剂量 |
5 mg/kg或20 mg/kg;口服;每天一次,每周6天,从第0天到第14天 |
应用 |
用5 mg/kg或 20 mg/kg的apilimod口服给药,在第14和18天降低了EAU(实验性自身免疫性葡萄膜视网膜炎)的严重程度。此外,用20 mg/kg apilimod治疗的小鼠通过组织病理学分析显示EAU的严重程度也显著降低。 |
注意事项 |
请测试所有化合物在室内的溶解度,实际溶解度和理论值可能略有不同。这是由实验系统的误差引起的,属于正常现象。 |
参考文献: | |
[1] . Keino H, et al. Therapeutic effect of the potent IL-12/IL-23 inhibitor STA-5326 on experimental autoimmune uveoretinitis. Arthritis Res Ther. 2008;10(5):R122. | |
[2].Wada Y, et al. Selective abrogation of Th1 response by STA-5326, a potent IL-12/IL-23 inhibitor. Blood. 2007 Feb 1;109(3):1156-64. |
质量控制和MSDS
- 批次: