Amphotericin B
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Amphotericin B是从链霉菌（Streptomyces nodosus）菌株产生的多烯类抗真菌抗生素。IC50 =0.028-0.290μg/ml
体外实验：Amphotericin B是治疗许多危及生命的真菌感染的有效药物。在表达TLR2和CD14的细胞中，Amphotericin B诱导信号转导和炎症细胞因子释放。在表达TLR2、CD14和衔接蛋白MyD88的原代鼠巨噬细胞和人细胞系中，Amphotericin B诱导NF-κB依赖性报告子活性和细胞因子释放，而细胞缺乏任何一个均对Amphotericin B没有反应。具有TLR4突变的细胞对Amphotericin B刺激的反应比表达正常TLR4的细胞反应弱[1]。Amphotericin B可与哺乳动物膜的主要固醇，即胆固醇相互作用，由于其具有相对高的毒性，限制了Amphotericin B的有用性[2]。在悬浮于等渗蔗糖溶液中的负载KCl的脂质体中，低浓度AmB（≤ 0.1 μM）诱导极化电位，表明K+渗透。大于0.1 μM AmB允许阳离子和阴离子运动。在悬浮在等渗NaCl溶液中的LPs中， 0.05 μM AmB导致负膜电位几乎完全崩溃，表明Na+进入细胞[3]。
在体实验：在仓鼠瘙痒病模型中，Amphotericin B延长了孵育时间，降低PrPSc积累。在具有传染性亚急性海绵状脑病（TSSE）的小鼠中，Amphotericin B显著降低PrPSc含量[4]。
参考文献：
[1]. Sau K1, Mambula SS, Latz E, Henneke P, Golenbock DT, Levitz SM. The antifungal drug amphotericin B promotes inflammatory cytokine release by a Toll-like receptor- and CD14-dependent mechanism. J Biol Chem. 2003 Sep 26;278(39):37561-8. Epub 2003 Jul 14.
[2]. Barwicz J1, Tancrède P.The effect of aggregation state of amphotericin-B on its interactions with cholesterol- or ergosterol-containing phosphatidylcholine monolayers. Chem Phys Lipids. 1997 Feb 28;85(2):145-55.
[3]. Ramos H1, Valdivieso E, Gamargo M, Dagger F, Cohen BE. Amphotericin B kills unicellular leishmanias by forming aqueous pores permeable to small cations and anions. J Membr Biol. 1996 Jul;152(1):65-75.
[4]. Demaimay R1, Adjou K, Lasmézas C, Lazarini F, Cherifi K,Seman M,Deslys JP, Dormont D. Pharmacological studies of a new derivative of amphotericin B, MS-8209, in mouse and hamster scrapie. J Gen Virol. 1994 Sep;75 ( Pt 9):2499-503.4. Demaimay R1, Adjou K, Lasmézas C, Lazarini F, Cherifi K,Seman M, Deslys JP, Dormont D. Pharmacological studies of a new derivative of amphotericin B, MS-8209, in mouse and hamster scrapie. J Gen Virol. 1994 Sep;75 ( Pt 9):2499-503.
- 1. Lukas Hafner, Maxime Pichon, et al. "Listeria monocytogenes faecal carriage is common and depends on the gut microbiota." Nat Commun. 2021 Nov 24;12(1):6826. PMID: 34819495
- 2. Xue-Ming Zhu , Lin Li, et al. "A VASt-domain protein regulates autophagy, membrane tension, and sterol homeostasis in rice blast fungus." Autophagy. 2020 Nov 11. PMID: 33176558
- 3. Perni S, Lavorato M, Beam KG. "De novo reconstitution reveals the proteins required for skeletal muscle voltage-induced Ca(2+) release." Proc Natl Acad Sci U S A. 2017 Dec 26;114(52):13822-13827. PMID: 29229815
| Storage | Store at -20°C |
| M.Wt | 924.08 |
| Cas No. | 1397-89-3 |
| Formula | C47H73NO17 |
| Solubility | ≥46.2 mg/mL in DMSO; insoluble in EtOH; insoluble in H2O |
| SDF | Download SDF |
| Canonical SMILES | O=C(C[C@@H](C[C@@H](CC[C@@H](O)[C@@H]1O)O)O)O[C@@H](C)[C@H](C)[C@H](O)[C@@H](C)/C=C/C=C/C=C/C=C/C=C/C=C/C=C/[C@H](O[C@H]2[C@@H](O)[C@@H](N)[C@H](O)[C@@H](C)O2)C[C@@H]([C@H](C(O)=O)[C@@H](O)C3)OC3(O)C[C@H](C1)O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| 细胞实验[1]: | |
|
细胞系 |
腹膜巨噬细胞; 表达TLR2和CD14的HEK293细胞 |
|
溶解方法 |
在DMSO中的溶解度>46.2mg/mL。为了获得更高的浓度，可以将离心管在37℃加热10分钟和/或在超声波浴中震荡一段时间。原液可以在-20℃以下储存几个月。 |
|
反应条件 |
1、2和4 μg/ml |
|
应用 |
在来自CD14敲除小鼠（C57BL/6 CD14 - / - ）和CD14野生型（C57BL/6 CD14 + / +）小鼠的腹膜巨噬细胞中，Amphotericin B无法诱导来自CD14 - / - 小鼠的巨噬细胞中TNF-α的产生。表达TLR2和CD14的HEK293细胞对Amphotericin B（1，2和4μg/ ml）的响应更强烈。 |
| 动物实验[2]: | |
|
动物模型 |
仓鼠瘙痒病模型 |
|
剂量 |
2.5 mg/kg; 接种后0-7天注射 |
|
应用 |
在瘙痒病大脑内感染的仓鼠中，Amphotericin B显著延长生存时间14.7天。 |
|
注意事项 |
请测试所有化合物在室内的溶解度，实际溶解度和理论值可能略有不同，这是由实验系统的误差引起的，属于正常现象。 |
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References: [1]. Sau K1,Mambula SS,Latz E,Henneke P,Golenbock DT,Levitz SM.The antifungal drug amphotericin B promotes inflammatory cytokine release by a Toll-like receptor- and CD14-dependent mechanism.J Biol Chem.2003 Sep 26;278(39):37561-8. Epub 2003 Jul 14. [2]. Demaimay R1,Adjou K,Lasmézas C,Lazarini F,Cherifi K,Seman M,Deslys JP,Dormont D.Pharmacological studies of a new derivative of amphotericin B, MS-8209, in mouse and hamster scrapie.J Gen Virol.1994 Sep;75 ( Pt 9):2499-503. |
|
质量控制和MSDS
- 批次:
化学结构
相关生物数据
相关生物数据
