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Daunorubicin HCl

现货
Catalog No.
B2285
DNA拓扑异构酶II抑制剂
组合的产品项目
规格价格库存 数量
10mM (in 1mL DMSO)
¥ 500.00
现货
10mg
¥ 500.00
现货
50mg
¥ 1,500.00
现货

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Background

Daunorubicin HCl is an inhibitor of DNA topoisomerase II [1].

Daunorubicin is an anthracycline antibiotic. It is also used as an effective chemotherapeutic agent against tumors especially acute myeloid leukaemia and acute lymphocytic leukaemia. Daunorubicin can affect the metabolism and synthesis of DNA and RNA. In the in vitro assay, daunorubicin inhibits the incorporation of thymidine and uridine into L1210 cells. It also inhibits the incorporation of labeled precursors into the isolated DNA and RNA from incubated cells. When treated with leukemic cells isolated from acute lymphocytic leukemia patients, daunorubicin significantly inhibits the biosynthesis of the DNA and RNA macromolecules [2, 3].

References:
[1] Hande K R. Etoposide: four decades of development of a topoisomerase II inhibitor. European Journal of Cancer, 1998, 34(10): 1514-1521.
[2] Momparler R L, Karon M, Siegel S E, et al. Effect of adriamycin on DNA, RNA, and protein synthesis in cell-free systems and intact cells. Cancer Research, 1976, 36(8): 2891-2895.
[3] Meriwether W D, Bachur N R. Inhibition of DNA and RNA metabolism by daunorubicin and adriamycin in L1210 mouse leukemia. Cancer research, 1972, 32(6): 1137-1142.

Chemical Properties

StorageStore at -20°C
M.Wt563.98
Cas No.23541-50-6
FormulaC27H30ClNO10
Solubility≥28.2mg/mL in DMSO
Chemical Name(8S,10S)-8-acetyl-10-(((2R,4S,5S,6S)-4-amino-5-hydroxy-6-methyltetrahydro-2H-pyran-2-yl)oxy)-6,8,11-trihydroxy-1-methoxy-7,8,9,10-tetrahydrotetracene-5,12-dione hydrochloride
SDFDownload SDF
Canonical SMILESCl[H].O=C(C([H])([H])[H])[C@@](C([H])([H])[C@]1([H])O[C@](C([H])([H])[C@]2([H])N([H])[H])([H])O[C@](C([H])([H])[H])([H])[C@@]2([H])O[H])(C([H])([H])C3=C1C(O[H])=C(C(C4=C5C([H])=C([H])C([H])=C4OC([H])([H])[H])=O)C(C5=O)=C3O[H])O[H]
运输条件试用装:蓝冰运输。 其他可选规格:常温运输或根据您的要求用蓝冰运输。
一般建议为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。

试验操作

细胞实验 [1]:

细胞系

小鼠黑色素瘤细胞(B16F10)和小鼠成纤维细胞NIH / 3T3

溶解方法

在DMSO的溶解度>28.2mg/mL。为了获得更高的浓度,可以将离心管在37℃加热10分钟和/或在超声波浴中震荡一段时间。原液可以在-20℃以下储存几个月。

反应条件

5至500μM,24小时

应用

15μM的daunorubicin能够对黑色素瘤细胞产生毒性。与未处理的细胞相比,daunorubicin对成纤维细胞造成轻微的形态改变,并观察到细胞密度的降低,抑制细胞增殖。

动物实验 [2]:

动物模型

Balb-c小鼠

剂量

单次腹膜内注射,2.3至28.25μmol/ kg

应用

在Balb-c小鼠中,较低剂量(1.8μmol/ kg)的daunorubicin处理组肿瘤生长抑制较轻微,而在较高剂量(7.5μmol/ kg)水平下,daunorubici处理下的肿瘤生长抑制很显著。所有来自盐水处理的动物都发生转移节点,而80%的daunorubicin处理组出现转移灶。

注意事项

请测试所有化合物在室内的溶解度,实际溶解度和理论值可能略有不同。这是由实验系统的误差引起的,属于正常现象。

References:

[1]. Contente TC, Kretzer IF., et al. Association of daunorubicin to a lipid nanoemulsion that binds to low-density lipoprotein receptors enhances the antitumour action and decreases the toxicity of the drug in melanoma-bearing mice. J Pharm Pharmacol. 2014 Dec;66(12):1698-709. doi: 10.1111/jphp.12296

质量控制

化学结构

Daunorubicin HCl