Plumbagin
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
IC50：作用于A549细胞的IC50值为11.69 μM。
Plumbagin是一种醌组分，分离自白花丹属的根，具有抗癌、抗动脉硬化和抗菌效果。
体外：Plumbagin通过诱导癌细胞经历G2/M期阻滞和细胞凋亡，发挥有效的细胞生长抑制。细胞周期阻滞与p21水平的增加以及Cdc2、CDC25C和细胞周期蛋白B1数量的减少有关。Plumbagin治疗增加失活的磷酸化Cdc2和cdc25C的水平。p53活性的阻断部分减少Plumbagin诱导的细胞凋亡和G2/M期阻滞，表明其可能是通过p53依赖性和非依赖性途径起作用[1]。
体内：为了确定Plumbagin是否抑制体内肿瘤生长，A549细胞注射入裸鼠。与赋形剂处理的小鼠对比，接受2 mg/kg/天剂量Plumbagin治疗的小鼠肿瘤生长抑制最为明显，其中观察到肿瘤体积减少约80%。通过监测体重判断，Plumbagin处理的小鼠未观察到毒性迹象[1]。
临床试验：N/A
参考文献：
[1] Hsu YL,Cho CY,Kuo PL,Huang YT,Lin CC. Plumbagin (5-hydroxy-2-methyl-1,4-naphthoquinone) induces apoptosis and cell cycle arrest in A549 cells through p53 accumulation via c-Jun NH2-terminal kinase-mediated phosphorylation at serine 15 in vitro and in vivo. J Pharmacol Exp Ther.2006 Aug;318(2):484-94.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 188.18 |
| Cas No. | 481-42-5 |
| Formula | C11H8O3 |
| Solubility | insoluble in H2O; ≥25.75 mg/mL in EtOH with ultrasonic; ≥6.35 mg/mL in DMSO |
| Chemical Name | 5-hydroxy-2-methylnaphthalene-1,4-dione |
| SDF | Download SDF |
| Canonical SMILES | CC1=CC(=O)C2=C(C1=O)C=CC=C2O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
