BMS-790052 dihydrochloride
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
BMS-790052是一种强效的HCV NS5A抑制剂，EC50值为9-50 pM [1]。
NS5A（非结构蛋白5A）是一种锌结合蛋白，并在HCV RNA复制中起关键作用。NS5A参与介导宿主细胞功能和HCV感染，因此被认为是一种用于治疗HCV的有希望的靶点[2]。
BMS-790052是一种选择性的HCV NS5A抑制剂，并具有迄今报道的最强效的抑制HCV复制的能力。使用FRET实验确定BMS-790052对HCV NS5A的疗效时，复制子细胞培养在96孔培养板中，12小时后加入BMS-790052以测试复制子细胞活性及细胞毒性，结果表明，BMS-790052对于基因型1a和1b的平均值分别为50和9 pM[1]。在具有GT-5a杂交复制子（GT-5A-6、GT-5A-7和GT-5A-9）的稳定复制细胞系中，BMS-790052治疗表现出有效的抗病毒活性，EC50值范围是3至7 pM [3]。
已经表明，用健康人员测试时，高达200 mg 的BMS-790052口服给药具有安全性和良好耐受性，用HCV感染患者测试时，100 mg剂量的BMS-790052具有安全性和良好耐受性[1]。
参考文献：
[1]. Gao, M., et al., Chemical genetics strategy identifies an HCV NS5A inhibitor with a potent clinical effect. Nature, 2010. 465(7294): p. 96-100.
[2]. Eyre, N.S. and M.R. Beard, HCV NS5A inhibitors disrupt replication factory formation: a novel mechanism of antiviral action. Gastroenterology, 2014. 147(5): p. 959-62.
[3]. Wang, C., et al., Comparison of daclatasvir resistance barriers on NS5A from hepatitis C virus genotypes 1 to 6: implications for cross-genotype activity. Antimicrob Agents Chemother, 2014. 58(9): p. 5155-63.
| Storage | Store at -20°C |
| M.Wt | 811.8 |
| Cas No. | 1009119-65-6 |
| Formula | C40H52Cl2N8O6 |
| Synonyms | Daclatasvir dihydrochloride;BMS790052 dihydrochloride;BMS 790052 dihydrochloride |
| Solubility | Soluble in DMSO |
| Chemical Name | dimethyl ((2S,2'S)-((2S,2'S)-2,2'-(5,5'-([1,1'-biphenyl]-4,4'-diyl)bis(1H-imidazole-5,2-diyl))bis(pyrrolidine-2,1-diyl))bis(3-methyl-1-oxobutane-2,1-diyl))dicarbamate dihydrochloride |
| SDF | Download SDF |
| Canonical SMILES | CC(C)[C@H](NC(OC)=O)C(N1[C@H](C2=NC=C(C3=CC=C(C4=CC=C(C5=CN=C([C@@H]6CCCN6C([C@@H](NC(OC)=O)C(C)C)=O)N5)C=C4)C=C3)N2)CCC1)=O.Cl.Cl |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
