Ferrostatin-1 (Fer-1)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Ferrostatin-1 是一种选择性的ferroptosis抑制剂。
Ferroptosis是一种受调控的、氧化的、非凋亡性细胞死亡,已发现Ferrostatin-1是其强效的抑制剂。通过阻断胱氨酸转运和谷胱甘肽产生,Fer-1可以抑制氧化的、铁-依赖性的癌细胞死亡。据报道,Fer-1通过抑制脂质过氧化防止亨廷顿氏病细胞模型的死亡。[ 1 ]
参考文献:
1. Skouta, R., et al., Ferrostatins inhibit oxidative lipid damage and cell death in diverse disease models. J Am Chem Soc, 2014. 136(12): p. 4551-6.
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Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 262.35 |
Cas No. | 347174-05-4 |
Formula | C15H22N2O2 |
Solubility | ≥149 mg/mL in DMSO; ≥99.6 mg/mL in EtOH with ultrasonic; insoluble in H2O |
SDF | Download SDF |
Canonical SMILES | NC1=C(NC2CCCCC2)C=CC(C(OCC)=O)=C1 |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
细胞实验 [1]: | |
细胞系 |
健康中等多刺神经元,少突胶质细胞,肾近端小管细胞 |
溶解方法 |
该化合物在DMSO中的溶解度大于9.8 mg/mL。若获取更高浓度的溶液,可在37℃下孵育10分钟,随后在超声波浴中摇匀。-20℃以下可储存数月。 |
反应条件 |
10 nM,100 nM,1 μM |
应用 |
Fer-1(10 nM、100 nM和1 μM)显著增加健康MSN的数量。Fer-1(1 μM)增加健康MSN的数量。Fer-1(100 nM)保护少突胶质细胞免于胱氨酸剥夺。Fer-1(0.1-2 μM)可防止羟基喹啉和硫酸亚铁铵引起的致死性,HQ和Fe使用量为各10 μM。 |
注意事项 |
由于实验环境的不同,实际溶解度可能与理论值略有不同,请测试室内所有化合物的溶解度。 |
References: [1]. Skouta R, Dixon S J, Wang J, et al. Ferrostatins inhibit oxidative lipid damage and cell death in diverse disease models[J]. Journal of the American Chemical Society, 2014, 136(12): 4551-4556. |
Description | Ferrostatin-1(Fer-1)是一种选择性的erastin诱导的ferroptosis抑制剂,EC50值为60 nM。 | |||||
靶点 | erastin induced ferroptosis | |||||
IC50 | 60 nM (EC50) |
质量控制和MSDS
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