In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Cucurbitacin E, a widely available plant-derived natural product, is a useful tool to study actin dynamics in cells and actin-based processes such as cytokinesis .
In vitro: In assays using pure fluorescently labeled actin, Cucurbitacin E affected depolymerization without affecting the polymerization. It inhibited actin depolymerization at substoichiometric concentrations up to 1:6 cucurbitacin E:actin. Cucurbitacin E specifically bound to F-actin to form a covalent bond at residue Cys257, but not to monomeric actin (G-actin) . In human leukemia HL-60 cells, Cucurbitacin E (3-50 nmol/l) inhibited the growth of HL-60 cells. At high concentrations (1-10 mol/l), Cucurbitacin E induced apoptosis of HL-60 cells and activation of caspase-3, 8 and 9. Jurkat leukemia cells with or without caspase-8 expression were nearly equally sensitive to cucurbitacin E-induced apoptosis. Cucurbitacin E disrupted the actin cytoskeleton. In a series of cucurbitacin analogues, the anti-proliferative activity was correlated with the disruption of the F-actin cytoskeleton directly .
. Sorensen P M, Iacob R E, Fritzsche M, et al. The natural product cucurbitacin E inhibits depolymerization of actin filaments[J]. ACS chemical biology, 2012, 7(9): 1502-1508.
. Li Y, Wang R, Ma E, et al. The induction of G2/M cell-cycle arrest and apoptosis by cucurbitacin E is associated with increased phosphorylation of eIF2α in leukemia cells[J]. Anti-cancer drugs, 2010, 21(4): 389-400.
. Duncan K L K, Duncan M D, Alley M C, et al. Cucurbitacin E-induced disruption of the actin and vimentin cytoskeleton in prostate carcinoma cells[J]. Biochemical pharmacology, 1996, 52(10): 1553-1560.
|Storage||Store at -20°C|
|Chemical Name||[(E,6R)-6-[(8S,9R,10R,13R,14S,16R,17R)-2,16-dihydroxy-4,4,9,13,14-pentamethyl-3,11-dioxo-8,10,12,15,16,17-hexahydro-7H-cyclopenta[a]phenanthren-17-yl]-6-hydroxy-2-methyl-5-oxohept-3-en-2-yl] acetate|