Cucurbitacin E
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Cucurbitacin E是广泛可用的植物来源的天然产物，是用于研究细胞中的肌动蛋白动力学和基于肌动蛋白的过程如胞质分裂的工具[1]。
体外实验：在使用纯的荧光标记的肌动蛋白的实验中，Cucurbitacin E特异性影响解聚而不影响聚合。在Cucurbitacin E：肌动蛋白的亚化学计量浓度高达1：6时，它抑制肌动蛋白解聚。Cucurbitacin E特异性结合F-肌动蛋白，与残基Cys257形成共价键，但不结合单体肌动蛋白（G-肌动蛋白）[1]。在人白血病HL-60细胞中，Cucurbitacin E（3-50 nmol/l）抑制HL-60细胞的生长。高浓度（1-10 mol/l）Cucurbitacin E诱导HL-60细胞凋亡和caspase-3、caspase-8和caspase-9的活化。具有或不具有caspase-8表达的Jurkat白血病细胞对Cucurbitacin E诱导的细胞凋亡具有几乎同等的敏感性[2]。Cucurbitacin E破坏肌动蛋白细胞骨架，在一系列葫芦素类似物中，抗增殖活性与F-肌动蛋白细胞骨架的破坏直接相关[3]。
参考文献：
[1]. Sorensen P M, Iacob R E, Fritzsche M, et al. The natural product cucurbitacin E inhibits depolymerization of actin filaments[J]. ACS chemical biology, 2012, 7(9): 1502-1508.
[2]. Li Y, Wang R, Ma E, et al. The induction of G2/M cell-cycle arrest and apoptosis by cucurbitacin E is associated with increased phosphorylation of eIF2α in leukemia cells[J]. Anti-cancer drugs, 2010, 21(4): 389-400.
[3]. Duncan K L K, Duncan M D, Alley M C, et al. Cucurbitacin E-induced disruption of the actin and vimentin cytoskeleton in prostate carcinoma cells[J]. Biochemical pharmacology, 1996, 52(10): 1553-1560.
| Storage | Store at -20°C |
| M.Wt | 556.69 |
| Cas No. | 18444-66-1 |
| Formula | C32H44O8 |
| Solubility | insoluble in EtOH; insoluble in H2O; ≥10.2 mg/mL in DMSO |
| Chemical Name | [(E,6R)-6-[(8S,9R,10R,13R,14S,16R,17R)-2,16-dihydroxy-4,4,9,13,14-pentamethyl-3,11-dioxo-8,10,12,15,16,17-hexahydro-7H-cyclopenta[a]phenanthren-17-yl]-6-hydroxy-2-methyl-5-oxohept-3-en-2-yl] acetate |
| SDF | Download SDF |
| Canonical SMILES | CC(=O)OC(C)(C)C=CC(=O)C(C)(C1C(CC2(C1(CC(=O)C3(C2CC=C4C3C=C(C(=O)C4(C)C)O)C)C)C)O)O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
