Caspase-3 Fluorometric Assay Kit
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
含半胱氨酸的天冬氨酸蛋白水解酶(Caspase) 是一个半胱氨酸蛋白酶家族,在细胞凋亡、坏死和炎症中起重要作用。 Caspase 的连续激活在细胞凋亡中起重要作用。 Caspase-3 是一种 caspase 蛋白,可切割和激活 caspase-6 和 -7,并被 caspase-8、-9 和 -10 加工和激活。 Caspase-3 是一种重要的caspase 蛋白,参与阿尔茨海默病相关的淀粉样 β4A 前体蛋白的裂解。 Caspase-3 识别四肽序列 D-x-x-D 并水解天冬氨酸残基后的肽键。
Caspase-3 Fluorometric Assay Kit 为检测 DEVD 依赖性 Caspase 活性提供了一种方便且简单的方法。 DEVD-AFC(AFC:7-氨基-4-三氟甲基香豆素)发出蓝光(λmax = 400 nm);当 Caspase-3 或相关 Caspase 裂解 DEVD-AFC 时,游离 AFC 会发出黄绿色荧光(λmax = 505 nm),可使用荧光微量滴定板读取器或荧光计对其进行量化。来自凋亡样品的 AFC 荧光与未诱导对照的比较确定了 Caspase-3 活性的增加倍数。
Features | Fast and convenient Simple one-step procedure; takes 1-2 hours. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-3/CPP32 activity. |
Shipping | Gel pack |
Storage Conditions | Store at -20°C. |
Species reactivity | Mammalian |
Applications | Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis. |
Kit components | Cell Lysis Buffer 2X Reaction Buffer DEVD-AFC (1 mM) DTT (1 M) |
- 1. Yao H, Fan M, et al. "Autophagy suppresses resveratrol-induced apoptosis in renal cell carcinoma 786-O cells." Oncol Lett. 2020;19(4):3269-3277. PMID:32256822
- 2. Ma C, Wang J, et al. "Therapeutic effects of bone mesenchymal stem cells on oral and maxillofacial defects: a novel signaling pathway involving miR-31/CXCR4/Akt axis." J Recept Signal Transduct Res. 2019 Oct 1:1-10. PMID:31573375
组分 | K2007-25 25 assays |
K2007-100 100 assays |
K2007-200 200 assays |
K2007-400 400 assays |
Part Number |
Cell Lysis Buffer | 25 ml | 100 ml | 100 ml | 100 ml | K2007-C-1 |
2 X Reaction Buffer | 2 ml | 4 x 2 ml | 16 ml | 32 ml | K2007-C-2 |
DEVD-AFC (1 mM) | 125 µl | 500 µl | 2 x 0.5 ml | 2 x 1 ml | K2007-C-3 |
DTT (1 M) | 100 µl | 400 µl | 400 µl | 400 µl | K2007-C-4 |
-20°C保存 |